RUFY1 Antibody (A-4): sc-398740
- RUFY1 Antibody (A-4) is a mouse monoclonal IgG2a κ RUFY1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 461-585 mapping within an internal region of RUFY1 of human origin
- RUFY1 Antibody (A-4) is recommended for detection of RUFY1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RUFY1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RUFY1 Antibody (A-4) for WB applications. These reagents are now offered in bundles with RUFY1 Antibody (A-4) (see ordering information below).
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RUFY1 Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects RUFY1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RUFY1 Antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RUFY1, also known as RUN and FYVE domain containing 1, RABIP4, or ZFYVE12, is a 708 amino acid protein that plays a crucial role in cellular processes by localizing to the cytoplasm and early endosome membrane. Such localization is vital for binding phosphatidylinositol 3-phosphate-containing vesicles, which are essential for early endosomal trafficking. RUFY1 contains one RUN domain and one FYVE-type zinc finger, enabling specific interaction with phosphatidylinositol 3-phosphate, thereby facilitating transport and sorting of endosomal cargo. RUFY1 undergoes post-translational modifications, including phosphorylation, which can occur upon DNA damage through ATM or ATR kinases. Phosphorylation of specific tyrosine residues, such as Tyr 389 and Tyr 400, is believed to be critical for proper localization to endosomes. Three isoforms of RUFY1 arise from alternative splicing, contributing to regulatory mechanisms and functional diversity in various tissues, including testis, lung, brain, and kidney.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RUFY1 Antibody (A-4) References:
- A FYVE-finger-containing protein, Rabip4, is a Rab4 effector involved in early endosomal traffic. | Cormont, M., et al. 2001. Proc Natl Acad Sci U S A. 98: 1637-42. PMID: 11172003
- Role of the FYVE finger and the RUN domain for the subcellular localization of Rabip4. | Mari, M., et al. 2001. J Biol Chem. 276: 42501-8. PMID: 11509568
- Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking. | Yang, J., et al. 2002. J Biol Chem. 277: 30219-26. PMID: 11877430
- Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes. | Fouraux, MA., et al. 2004. Mol Biol Cell. 15: 611-24. PMID: 14617813
- Characterization of RUSC1 and RUSC2 genes in silico. | Katoh, M. and Katoh, M. 2004. Oncol Rep. 12: 933-8. PMID: 15375525
- The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes. | Mari, M., et al. 2006. J Cell Sci. 119: 1297-306. PMID: 16522682
- The Rab4A effector protein Rabip4 is involved in migration of NIH 3T3 fibroblasts. | Vukmirica, J., et al. 2006. J Biol Chem. 281: 36360-8. PMID: 17001082
- Evolutionarily conserved structural and functional roles of the FYVE domain. | Hayakawa, A., et al. 2007. Biochem Soc Symp. 95-105. PMID: 17233583
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RUFY1 Antibody (A-4) | sc-398740 | 200 µg/ml | $322.00 | |||
RUFY1 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-530911 | 200 µg Ab; 10 µg BP | $361.00 | |||
RUFY1 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-524465 | 200 µg Ab, 40 µg BP | $361.00 | |||
RUFY1 Antibody (A-4): m-IgG2a BP-HRP Bundle | sc-547626 | 200 µg Ab; 10 µg BP | $361.00 | |||
RUFY1 Antibody (A-4) AC | sc-398740 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RUFY1 Antibody (A-4) HRP | sc-398740 HRP | 200 µg/ml | $322.00 | |||
RUFY1 Antibody (A-4) FITC | sc-398740 FITC | 200 µg/ml | $336.00 | |||
RUFY1 Antibody (A-4) PE | sc-398740 PE | 200 µg/ml | $349.00 | |||
RUFY1 Antibody (A-4) Alexa Fluor® 488 | sc-398740 AF488 | 200 µg/ml | $364.00 | |||
RUFY1 Antibody (A-4) Alexa Fluor® 546 | sc-398740 AF546 | 200 µg/ml | $364.00 | |||
RUFY1 Antibody (A-4) Alexa Fluor® 594 | sc-398740 AF594 | 200 µg/ml | $364.00 | |||
RUFY1 Antibody (A-4) Alexa Fluor® 647 | sc-398740 AF647 | 200 µg/ml | $364.00 | |||
RUFY1 Antibody (A-4) Alexa Fluor® 680 | sc-398740 AF680 | 200 µg/ml | $364.00 | |||
RUFY1 Antibody (A-4) Alexa Fluor® 790 | sc-398740 AF790 | 200 µg/ml | $364.00 |