Rpp40 Antibody (G-4): sc-515079
- Rpp40 Antibody (G-4) is a mouse monoclonal IgG2a κ Rpp40 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Rpp40 of human origin
- Rpp40 Antibody (G-4) is recommended for detection of Rpp40 of human origin by WB, IP, IF and ELISA
- Anti-Rpp40 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-515079 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rpp40 Antibody (G-4) for WB applications. These reagents are now offered in bundles with Rpp40 Antibody (G-4) (see ordering information below).
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Rpp40 Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rpp40 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rpp40 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rpp40, also known as ribonuclease P protein subunit p40 or RNASEP1, is a crucial component of the ribonuclease P complex, which is responsible for removing 5-prime leader sequences from precursor tRNA molecules, a vital step in tRNA maturation and protein synthesis. This protein consists of 363 amino acids and is primarily localized in the nucleolus, where Rpp40 plays a significant role in the processing of tRNA and other RNA species. The Rpp40 gene is highly conserved across various species, including chimpanzees, dogs, cows, mice, rats, chickens, and zebrafish, and is located on human chromosome 6p25.1, a region that harbors approximately 1,200 genes and is associated with critical biological functions, including immune response and neurodegenerative diseases. The presence of Rpp40 and its interaction with other ribonuclease P proteins, such as Rpp14, Rpp20, Rpp25, Rpp29, Rpp30, and Rpp38, underscores its importance in maintaining cellular RNA integrity and function, making anti-Rpp40 antibody (G-4) an essential tool for researchers studying RNA processing and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rpp40 Antibody (G-4) References:
- Rpp14 and Rpp29, two protein subunits of human ribonuclease P. | Jarrous, N., et al. 1999. RNA. 5: 153-7. PMID: 10024167
- Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P. | Jarrous, N., et al. 1999. J Cell Biol. 146: 559-72. PMID: 10444065
- Protein-RNA interactions in the subunits of human nuclear RNase P. | Jiang, T., et al. 2001. RNA. 7: 937-41. PMID: 11455963
- The DNA sequence and analysis of human chromosome 6. | Mungall, AJ., et al. 2003. Nature. 425: 805-11. PMID: 14574404
- Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex. | Welting, TJ., et al. 2004. Nucleic Acids Res. 32: 2138-46. PMID: 15096576
- Inhibition of the expression of the human RNase P protein subunits Rpp21, Rpp25, Rpp29 by external guide sequences (EGSs) and siRNA. | Zhang, H. and Altman, S. 2004. J Mol Biol. 342: 1077-83. PMID: 15351636
- Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. | Jarrous, N., et al. 1998. RNA. 4: 407-17. PMID: 9630247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rpp40 Antibody (G-4) | sc-515079 | 200 µg/ml | $316.00 | |||
Rpp40 Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-531283 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rpp40 Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-524929 | 200 µg Ab, 40 µg BP | $354.00 | |||
Rpp40 Antibody (G-4): m-IgG2a BP-HRP Bundle | sc-547751 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rpp40 Antibody (G-4) X | sc-515079 X | 200 µg/0.1 ml | $316.00 | |||
Rpp40 Antibody (G-4) AC | sc-515079 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Rpp40 Antibody (G-4) HRP | sc-515079 HRP | 200 µg/ml | $316.00 | |||
Rpp40 Antibody (G-4) FITC | sc-515079 FITC | 200 µg/ml | $330.00 | |||
Rpp40 Antibody (G-4) PE | sc-515079 PE | 200 µg/ml | $343.00 | |||
Rpp40 Antibody (G-4) Alexa Fluor® 488 | sc-515079 AF488 | 200 µg/ml | $357.00 | |||
Rpp40 Antibody (G-4) Alexa Fluor® 546 | sc-515079 AF546 | 200 µg/ml | $357.00 | |||
Rpp40 Antibody (G-4) Alexa Fluor® 594 | sc-515079 AF594 | 200 µg/ml | $357.00 | |||
Rpp40 Antibody (G-4) Alexa Fluor® 647 | sc-515079 AF647 | 200 µg/ml | $357.00 | |||
Rpp40 Antibody (G-4) Alexa Fluor® 680 | sc-515079 AF680 | 200 µg/ml | $357.00 | |||
Rpp40 Antibody (G-4) Alexa Fluor® 790 | sc-515079 AF790 | 200 µg/ml | $357.00 |