RPE65 Antibody (E-5): sc-390787

RPE65 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects RPE65 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RPE65 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPE65, a member of the beta-carotene dioxygenase family, plays a crucial role in the visual cycle by facilitating the conversion of all-trans-retinyl esters to 11-cis-retinal, which is essential for the regeneration of visual pigments in photoreceptor cells. This process is vital for maintaining vision, as it allows for the proper functioning of the phototransduction cascade. RPE65 is primarily located in the retinal pigment epithelium, where RPE65 is anchored to the membrane via a lipid modification known as palmitoylation; this membrane-bound form is critical for enzymatic activity. Mutations in the RPE65 gene can lead to severe retinal diseases, including autosomal dominant retinitis pigmentosa and Leber congenital amaurosis type 2, highlighting the importance of RPE65 in ocular health and vision preservation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RPE65 Antibody (E-5) References:

1. Genetics and phenotypes of RPE65 mutations in inherited retinal degeneration.  |  Thompson, DA., et al. 2000. Invest Ophthalmol Vis Sci. 41: 4293-9. PMID: 11095629
2. New views on RPE65 deficiency: the rod system is the source of vision in a mouse model of Leber congenital amaurosis.  |  Seeliger, MW., et al. 2001. Nat Genet. 29: 70-4. PMID: 11528395
3. Correlation of regenerable opsin with rod ERG signal in Rpe65-/- mice during development and aging.  |  Rohrer, B., et al. 2003. Invest Ophthalmol Vis Sci. 44: 310-5. PMID: 12506090
4. A palmitoylation switch mechanism in the regulation of the visual cycle.  |  Xue, L., et al. 2004. Cell. 117: 761-71. PMID: 15186777
5. Functional Rescue of Retinal Degeneration-Associated Mutant RPE65 Proteins.  |  Jin, M., et al. 2016. Adv Exp Med Biol. 854: 525-32. PMID: 26427455
6. RPE65 and the Accumulation of Retinyl Esters in Mouse Retinal Pigment Epithelium.  |  Sheridan, C., et al. 2017. Photochem Photobiol. 93: 844-848. PMID: 28500718
7. Properties and Therapeutic Implications of an Enigmatic D477G RPE65 Variant Associated with Autosomal Dominant Retinitis Pigmentosa.  |  Kiang, AS., et al. 2020. Genes (Basel). 11: PMID: 33261050
8. A Comprehensive Study of the Retinal Phenotype of Rpe65-Deficient Dogs.  |  Annear, MJ., et al. 2021. Cells. 10: PMID: 33435495
9. An inducible amphipathic α-helix mediates subcellular targeting and membrane binding of RPE65.  |  Uppal, S., et al. 2023. Life Sci Alliance. 6: PMID: 36265895
10. The gene for the retinal pigment epithelium-specific protein RPE65 is localized to human 1p31 and mouse 3.  |  Hamel, CP., et al. 1994. Genomics. 20: 509-12. PMID: 8034329
11. Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro.  |  Hamel, CP., et al. 1993. J Biol Chem. 268: 15751-7. PMID: 8340400
12. Mutations in the RPE65 gene in patients with autosomal recessive retinitis pigmentosa or leber congenital amaurosis.  |  Morimura, H., et al. 1998. Proc Natl Acad Sci U S A. 95: 3088-93. PMID: 9501220

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RPE65 Antibody (E-5) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-RPE65 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RPE65 Antibody (E-5) Specifications

Alternate Product Name: Retinol Isomerase

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 445-470

Epitope Target: RPE65

Epitope Species: human

Product Reactivity/Detection: RPE65

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for RPE65 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin