Rootletin Antibody (E-5): sc-390720

Rootletin Antibody (E-5) is a mouse monoclonal IgG1 antibody that detects Rootletin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rootletin, also known as CROCC (ciliary rootlet coiled-coil protein), is a 2017 amino acid protein that plays a crucial role in the formation of centriole-associated fibrous structures, serving as an essential component of the ciliary rootlet. Rootletin is primarily localized to basal bodies and centrosomes in both ciliated and nonciliated cells, where Rootletin associates with the proximal ends of basal bodies. In photoreceptors, Rootletin is vital for forming elongated polymers between centrioles, which is important for maintaining the structural integrity and function of cilia. Additionally, Rootletin is required for centrosome cohesion and interacts with C-Nap1, a centrosomal protein that regulates the linkage of centrioles to basal bodies. Rootletin exists as a homopolymer, and Rootletin′s association with centrosomes can be modulated through phosphorylation by Nek2 (NIMA-related kinase 2), highlighting the significance of post-translational modifications in Rootletin′s function. Furthermore, two isoforms of Rootletin arise from alternative splicing events, adding another layer of complexity to Rootletin′s regulation and function in cellular processes.

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Rootletin Antibody (E-5) References:

1. Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet.  |  Yang, J., et al. 2002. J Cell Biol. 159: 431-40. PMID: 12427867
2. Rootletin forms centriole-associated filaments and functions in centrosome cohesion.  |  Bahe, S., et al. 2005. J Cell Biol. 171: 27-33. PMID: 16203858
3. Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells.  |  Yang, J., et al. 2006. Mol Biol Cell. 17: 1033-40. PMID: 16339073
4. C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis.  |  Conroy, PC., et al. 2012. Cell Cycle. 11: 3769-78. PMID: 23070519
5. Striated rootlet and nonfilamentous forms of rootletin maintain ciliary function.  |  Mohan, S., et al. 2013. Curr Biol. 23: 2016-22. PMID: 24094853
6. SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation.  |  Akiyama, T., et al. 2017. Sci Rep. 7: 39967. PMID: 28059168
7. STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles.  |  Vlijm, R., et al. 2018. Proc Natl Acad Sci U S A. 115: E2246-E2253. PMID: 29463719
8. CROCC-mutated rhabdoid colorectal carcinoma showing in intercellular spaces lamellipodia and cellular projections revealed by electron microscopy.  |  Remo, A., et al. 2019. Virchows Arch. 475: 245-249. PMID: 30852641
9. Up-regulated microRNA-33b inhibits epithelial-mesenchymal transition in gallbladder cancer through down-regulating CROCC.  |  Xu, G., et al. 2020. Biosci Rep. 40: PMID: 31799620
10. Loss of Primary Cilia Potentiates BRAF/MAPK Pathway Activation in Rhabdoid Colorectal Carcinoma: A Series of 21 Cases Showing Ciliary Rootlet CoiledCoil (CROCC) Alterations.  |  Remo, A., et al. 2023. Genes (Basel). 14: PMID: 37239344

The preferred Rootletin antibody is Rootletin Antibody (C-2): sc-374056.