Rootletin Antibody (C-2): sc-374056

Rootletin Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rootletin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Rootletin antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rootletin, also known as CROCC (ciliary rootlet coiled-coil protein), is a 2017 amino acid protein that plays a crucial role in forming centriole-associated fibrous structures, serving as an essential component of the ciliary rootlet. Rootletin′s localization to basal bodies and centrosomes in both ciliated and nonciliated cells is vital for maintaining cellular organization and function. Specifically, Rootletin associates with proximal ends of basal bodies and forms elongated polymers between them in photoreceptors, which is critical for structural integrity and function of cilia. Furthermore, Rootletin is required for centrosome cohesion and interacts with C-Nap1, a centrosomal protein that regulates linkage of centrioles to basal bodies. Rootletin exists as a homopolymer, and association with centrosomes can be modulated through phosphorylation by Nek2 (NIMA-related kinase 2), highlighting importance of post-translational modifications in function. Additionally, two isoforms of Rootletin arise from alternative splicing events, further diversifying functional roles in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rootletin Antibody (C-2) References:

1. Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet.  |  Yang, J., et al. 2002. J Cell Biol. 159: 431-40. PMID: 12427867
2. The ciliary rootlet maintains long-term stability of sensory cilia.  |  Yang, J., et al. 2005. Mol Cell Biol. 25: 4129-37. PMID: 15870283
3. Rootletin forms centriole-associated filaments and functions in centrosome cohesion.  |  Bahe, S., et al. 2005. J Cell Biol. 171: 27-33. PMID: 16203858
4. Focus on molecules: rootletin.  |  Yang, J. and Li, T. 2006. Exp Eye Res. 83: 1-2. PMID: 16318850
5. Rootletin interacts with C-Nap1 and may function as a physical linker between the pair of centrioles/basal bodies in cells.  |  Yang, J., et al. 2006. Mol Biol Cell. 17: 1033-40. PMID: 16339073
6. Protein phosphatase-1alpha regulates centrosome splitting through Nek2.  |  Mi, J., et al. 2007. Cancer Res. 67: 1082-9. PMID: 17283141
7. Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion.  |  Graser, S., et al. 2007. J Cell Sci. 120: 4321-31. PMID: 18042621
8. beta-Catenin is a Nek2 substrate involved in centrosome separation.  |  Bahmanyar, S., et al. 2008. Genes Dev. 22: 91-105. PMID: 18086858
9. Rootletin prevents Cep68 from VHL-mediated proteasomal degradation to maintain centrosome cohesion.  |  Yin, H., et al. 2017. Biochim Biophys Acta Mol Cell Res. 1864: 645-654. PMID: 28089774
10. Cep44 functions in centrosome cohesion by stabilizing rootletin.  |  Hossain, D., et al. 2020. J Cell Sci. 133: PMID: 31974111
11. Identification of a Structurally Dynamic Domain for Oligomer Formation in Rootletin.  |  Ko, D., et al. 2020. J Mol Biol. 432: 3915-3932. PMID: 32325071
12. Roles for ELMOD2 and Rootletin in ciliogenesis.  |  Turn, RE., et al. 2021. Mol Biol Cell. 32: 800-822. PMID: 33596093

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rootletin Antibody (C-2) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Rootletin Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rootletin Antibody (C-2) Specifications

Alternate Product Name: Rootletin

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1882-1917

Epitope Target: Rootletin

Epitope Species: human

Product Reactivity/Detection: Rootletin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Rootletin Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin