RNH1 Antibody (C-1): sc-515561

RNH1 Antibody (C-1) is a mouse monoclonal IgG2b antibody that detects RNH1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RNH1, known as the placental ribonuclease inhibitor, is a crucial acidic protein composed of 460 amino acids, characterized by a notably high content of leucine and cysteine residues. RNH1 plays a vital role in regulating mRNA turnover and is a member of a family of cytoplasmic RNase inhibitors that are widely expressed across different tissues. RNH1 functions by binding to a diverse array of mammalian ribonucleases, effectively holding them in a latent form, which is essential for maintaining cellular RNA stability. Additionally, RNH1 is significant in angiogenesis, as RNH1 reversibly binds to angiogenin, a member of the RNaseA superfamily, thereby inhibiting the formation of new blood vessels. This inhibition is particularly important in cancer biology, as angiogenesis is a critical process for tumor growth and metastasis, suggesting RNH1 may have therapeutic implications in cancer gene therapy. With an ability to modulate RNase activity and influence angiogenic processes, anti-RNH1 antibody (C-1) serves as a valuable tool for researchers investigating RNA metabolism and cancer progression.

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RNH1 Antibody (C-1) References:

1. Molecular recognition of human eosinophil-derived neurotoxin (RNase 2) by placental ribonuclease inhibitor.  |  Iyer, S., et al. 2005. J Mol Biol. 347: 637-55. PMID: 15755456
2. Angiogenin cleaves tRNA and promotes stress-induced translational repression.  |  Yamasaki, S., et al. 2009. J Cell Biol. 185: 35-42. PMID: 19332886
3. Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.  |  Stephens, AN., et al. 2010. J Proteome Res. 9: 2438-49. PMID: 20199104
4. Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential.  |  Cremer, C., et al. 2015. J Immunother. 38: 85-95. PMID: 25710248
5. MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts.  |  Chang, EY., et al. 2019. Nat Commun. 10: 4265. PMID: 31537797
6. The cell and stress-specific canonical and noncanonical tRNA cleavage.  |  Rashad, S., et al. 2021. J Cell Physiol. 236: 3710-3724. PMID: 33043995
7. Non-enzymatic glycation of human angiogenin: Effects on enzymatic activity and binding to hRI and DNA.  |  Panda, A., et al. 2023. Biochimie. 208: 151-159. PMID: 36592684
8. Vascularized characteristics and functional regeneration of three-dimensional cell reconstruction of oral mucosa equivalents based on vascular homeostasis phenotypic modification.  |  Shi, L., et al. 2024. J Tissue Eng. 15: 20417314241268912. PMID: 39301507
9. The ribonuclease activity of nucleolar protein B23.  |  Herrera, JE., et al. 1995. Nucleic Acids Res. 23: 3974-9. PMID: 7479045
10. Ribonuclease inhibitor protein of human erythrocytes: characterization, loss of activity in response to oxidative stress, and association with Heinz bodies.  |  Moenner, M., et al. 1998. Blood Cells Mol Dis. 24: 149-64. PMID: 9628852

The preferred RNH1 antibody is RNH1 Antibody (A-9): sc-365783.