RNH1 Antibody (A-9): sc-365783

RNH1 Antibody (A-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects RNH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RNH1 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNH1, known as the placental ribonuclease inhibitor, is a crucial 460-amino acid protein characterized by a high content of leucine and cysteine residues, which contributes to structural stability and functional versatility. RNH1 is primarily located in the cytoplasm, where RNH1 plays a vital role in regulating ribonuclease activity, thereby controlling mRNA turnover and influencing gene expression. By binding to a wide range of mammalian ribonucleases, RNH1 maintains them in an inactive state, preventing unwanted RNA degradation. Additionally, RNH1 inhibits angiogenesis by reversibly binding to angiogenin, a member of the RNaseA superfamily, which is essential for tumor growth and metastasis. RNH1 may have significant implications in cancer gene therapy, making anti-RNH1 antibody (A-9) an invaluable tool for researchers studying RNA metabolism and cancer progression.

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RNH1 Antibody (A-9) References:

1. The Crithidia fasciculata RNH1 gene encodes both nuclear and mitochondrial isoforms of RNase H.  |  Engel, ML., et al. 2001. Nucleic Acids Res. 29: 725-31. PMID: 11160895
2. [Anti-tumor effect through human endostatin gene transfer in mice bearing B16 melanoma].  |  Zhang, B., et al. 2002. Zhonghua Zhong Liu Za Zhi. 24: 451-4. PMID: 12485496
3. Anti-tumor effect of hematopoietic cells carrying the gene of ribonuclease inhibitor.  |  Fu, P., et al. 2005. Cancer Gene Ther. 12: 268-75. PMID: 15592448
4. Molecular recognition of human eosinophil-derived neurotoxin (RNase 2) by placental ribonuclease inhibitor.  |  Iyer, S., et al. 2005. J Mol Biol. 347: 637-55. PMID: 15755456
5. Antitumor effects of human ribonuclease inhibitor gene transfected on B16 melanoma cells.  |  Chen, JX., et al. 2005. Int J Biochem Cell Biol. 37: 1219-31. PMID: 15778086
6. Inhibition of B16 melanoma growth in vivo by retroviral vector-mediated human ribonuclease inhibitor.  |  Wang, T., et al. 2005. Angiogenesis. 8: 73-81. PMID: 16132620
7. Ribonuclease inhibitor: structure and function.  |  Dickson, KA., et al. 2005. Prog Nucleic Acid Res Mol Biol. 80: 349-74. PMID: 16164979
8. Disruption of shape-complementarity markers to create cytotoxic variants of ribonuclease A.  |  Rutkoski, TJ., et al. 2005. J Mol Biol. 354: 41-54. PMID: 16188273
9. Gene therapeutic exploration: retrovirus-mediated soluble vascular endothelial growth factor receptor-2 (sFLK-1) inhibits the tumorigenicity of S180, MCF-7, and B16 cells in vivo.  |  Kou, B., et al. 2005. Oncol Res. 15: 239-47. PMID: 16261843
10. PTEN modulates miR-21 processing via RNA-regulatory protein RNH1.  |  Kim, YJ., et al. 2011. PLoS One. 6: e28308. PMID: 22162762
11. Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.  |  Parajuli, S., et al. 2017. J Biol Chem. 292: 15216-15224. PMID: 28717002
12. Molecular recognition of human angiogenin by placental ribonuclease inhibitor--an X-ray crystallographic study at 2.0 A resolution.  |  Papageorgiou, AC., et al. 1997. EMBO J. 16: 5162-77. PMID: 9311977

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNH1 Antibody (A-9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-RNH1 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNH1 Antibody (A-9) Specifications

Alternate Product Name: RNH1

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-135

Epitope Target: RNH1

Epitope Species: human

Product Reactivity/Detection: RNH1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for RNH1 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin