RNF5 Antibody (22B3): sc-81716

RNF5 Antibody (22B3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNF5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunohistochemistry. anti-RNF5 antibody (22B3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNF5, also known as ring finger protein 5, RMA1, NG2, or G16, is a 180 amino acid protein characterized by the presence of a RING-type zinc finger motif, which is crucial for function as a ubiquitin ligase. This motif allows RNF5 to bind two zinc atoms and is essential for a role in the ubiquitination pathway, a key mechanism in protein degradation. RNF5 is predominantly localized to the membrane and plays a significant role in regulating cell motility by modulating the ubiquitination of paxillin, a focal adhesion phosphoprotein that interacts with the cytoskeleton. By targeting paxillin for ubiquitination, RNF5 alters localization and affects ability to recruit signaling molecules to focal adhesions, thereby influencing cellular signaling and movement. Notably, while overexpression of RNF5 has been linked to the progression of breast cancer, reduced expression levels have been observed in muscular disorders, highlighting critical involvement in the regulation of cytoskeletal dynamics and potential implications in various diseases.

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RNF5 Antibody (22B3) References:

1. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.  |  Lorick, KL., et al. 1999. Proc Natl Acad Sci U S A. 96: 11364-9. PMID: 10500182
2. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.  |  Zhang, QH., et al. 2000. Genome Res. 10: 1546-60. PMID: 11042152
3. Rma1, a novel type of RING finger protein conserved from Arabidopsis to human, is a membrane-bound ubiquitin ligase.  |  Matsuda, N., et al. 2001. J Cell Sci. 114: 1949-57. PMID: 11329381
4. RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization.  |  Didier, C., et al. 2003. Mol Cell Biol. 23: 5331-45. PMID: 12861019
5. Sequential quality-control checkpoints triage misfolded cystic fibrosis transmembrane conductance regulator.  |  Younger, JM., et al. 2006. Cell. 126: 571-82. PMID: 16901789
6. Increased expression of the E3 ubiquitin ligase RNF5 is associated with decreased survival in breast cancer.  |  Bromberg, KD., et al. 2007. Cancer Res. 67: 8172-9. PMID: 17804730
7. The ER-bound RING finger protein 5 (RNF5/RMA1) causes degenerative myopathy in transgenic mice and is deregulated in inclusion body myositis.  |  Delaunay, A., et al. 2008. PLoS One. 3: e1609. PMID: 18270596
8. Pseudorabies virus tegument protein UL13 recruits RNF5 to inhibit STING-mediated antiviral immunity.  |  Kong, Z., et al. 2022. PLoS Pathog. 18: e1010544. PMID: 35584187
9. E3 ubiquitin ligase RNF5 attenuates pathological cardiac hypertrophy through STING.  |  Yang, LL., et al. 2022. Cell Death Dis. 13: 889. PMID: 36270989
10. The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation.  |  Li, Z., et al. 2023. Signal Transduct Target Ther. 8: 53. PMID: 36737599
11. The RING finger domain: a recent example of a sequence-structure family.  |  Borden, KL. and Freemont, PS. 1996. Curr Opin Struct Biol. 6: 395-401. PMID: 8804826
12. Cloning, expression and mapping of a novel RING-finger gene (RNF5), a human homologue of a putative zinc-finger gene from Caenorhabditis elegans.  |  Kyushiki, H., et al. 1997. Cytogenet Cell Genet. 79: 114-7. PMID: 9533025

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNF5 Antibody (22B3) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-RNF5 Antibody (22B3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNF5 Antibody (22B3) Specifications

Alternate Product Name: RNF5

Product Clone Name: 22B3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: RNF5

Epitope Species: human

Product Reactivity/Detection: RNF5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for RNF5 Antibody (22B3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin