RNF25 Antibody (C-12): sc-514944

RNF25 Antibody (C-12) is a mouse monoclonal IgG1 antibody that detects RNF25 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNF25, also known as RING finger protein 25 or AO7, is a 459 amino acid protein characterized by the presence of one RWD domain and one RING-type zinc finger, which is crucial for function as an E3 ubiquitin-protein ligase. The RING finger domain of RNF25 facilitates interaction with ubiquitin-conjugating enzymes, enabling ubiquitination of target proteins, a vital process for regulating protein degradation and maintaining cellular homeostasis. This ubiquitination mechanism is essential for controlling levels of short-lived or abnormal proteins, thereby influencing various cellular processes, including cell cycle regulation and apoptosis. Additionally, RNF25 has been implicated in modulating transcriptional activity of NFκB p65, a key player in inflammatory responses and cell survival, highlighting importance in both normal physiology and disease states. Anti-RNF25 antibody (C-12) is an invaluable tool for researchers studying the intricate roles of ubiquitination and impact on cellular functions.

The preferred RNF25 antibody is RNF25 Antibody (E-10): sc-398749.