RNF25 Antibody (E-10): sc-398749
- RNF25 Antibody (E-10) is a mouse monoclonal IgG3 κ RNF25 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 74-103 within an internal region of RNF25 of human origin
- recommended for detection of RNF25 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-398749 X, 200 µg/0.1 ml)
- m-IgG3 BP-HRP is the preferred secondary detection reagent for RNF25 Antibody (E-10) for WB applications. This reagent is now offered in a bundle with RNF25 Antibody (E-10) (see ordering information below).
RNF25 Antibody (E-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects RNF25 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RNF25 antibody (E-10) is available as the non-conjugated form. RNF25, also known as RING finger protein 25 or AO7, is a 459 amino acid protein characterized by the presence of one RWD domain and one RING-type zinc finger. The RING-type zinc finger is crucial as RNF25 facilitates E2-dependent E3 ubiquitin-protein ligase activity, which is essential for the ubiquitination process. Ubiquitination serves as a vital regulatory mechanism that targets short-lived or abnormal proteins for degradation, thereby maintaining cellular homeostasis and regulating various biological processes. RNF25′s ability to interact with E2 ligases allows specific target protein ubiquitination, influencing stability and function. Additionally, RNF25 may play a role in modulating NFκB p65 transcriptional activity, a key player in cell survival, inflammation, and immune responses. RNF25′s versatility in these critical cellular functions underscores its importance in both normal physiology and disease states, making RNF25 (E-10) antibody a valuable tool for researchers studying ubiquitination and its implications in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNF25 Antibody (E-10) References:
- RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. | Lorick, KL., et al. 1999. Proc Natl Acad Sci U S A. 96: 11364-9. PMID: 10500182
- RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit. | Asamitsu, K., et al. 2003. J Biol Chem. 278: 26879-87. PMID: 12748188
- Functional proteomics mapping of a human signaling pathway. | Colland, F., et al. 2004. Genome Res. 14: 1324-32. PMID: 15231748
- Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. | Penengo, L., et al. 2006. Cell. 124: 1183-95. PMID: 16499958
- The RING finger domain: a recent example of a sequence-structure family. | Borden, KL. and Freemont, PS. 1996. Curr Opin Struct Biol. 6: 395-401. PMID: 8804826
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF25 Antibody (E-10) | sc-398749 | 200 µg/ml | $316.00 | |||
RNF25 Antibody (E-10): m-IgG3 BP-HRP Bundle | sc-550603 | 200 µg Ab; 40 µg BP | $354.00 | |||
RNF25 (E-10) Neutralizing Peptide | sc-398749 P | 100 µg/0.5 ml | $68.00 | |||
RNF25 Antibody (E-10) X | sc-398749 X | 200 µg/0.1 ml | $316.00 |