RNF219 Antibody (A-4): sc-514812
- RNF219 Antibody (A-4) is a mouse monoclonal IgG1 κ RNF219 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-160 mapping at the N-terminus of RNF219 of human origin
- RNF219 Antibody (A-4) is recommended for detection of RNF219 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RNF219 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RNF219 Antibody (A-4) for WB applications. These reagents are now offered in bundles with RNF219 Antibody (A-4) (see ordering information below).
RNF219 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNF219 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNF219 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. RNF219 protein, consisting of 726 amino acids, features a RING-type zinc finger motif, which is crucial for its role in the ubiquitination pathway, a key mechanism for protein degradation and cellular regulation. This motif allows RNF219 to bind two zinc ions, facilitating interaction with other proteins involved in transcriptional regulation and cellular response to DNA damage. Notably, RNF219 undergoes phosphorylation in response to DNA damage, likely mediated by ATM or ATR kinases, highlighting importance in maintaining genomic stability. The gene encoding RNF219 is located on human chromosome 13, a region that harbors over 400 genes, including critical tumor suppressor genes like BRCA2 and RB1. Understanding RNF219 function and interactions is vital, as dysregulation can contribute to various diseases, including cancer, making RNF219 a significant target for research and therapeutic development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNF219 Antibody (A-4) References:
- RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. | Lorick, KL., et al. 1999. Proc Natl Acad Sci U S A. 96: 11364-9. PMID: 10500182
- Non-disjunction of chromosome 13. | Bugge, M., et al. 2007. Hum Mol Genet. 16: 2004-10. PMID: 17584770
- Variable expressivity in Patau syndrome is not all related to trisomy 13 mosaicism. | Hsu, HF. and Hou, JW. 2007. Am J Med Genet A. 143A: 1739-48. PMID: 17603803
- The origin of trisomy 13. | Hall, HE., et al. 2007. Am J Med Genet A. 143A: 2242-8. PMID: 17853475
- The RING finger. A novel protein sequence motif related to the zinc finger. | Freemont, PS. 1993. Ann N Y Acad Sci. 684: 174-92. PMID: 8317827
- The RING finger domain: a recent example of a sequence-structure family. | Borden, KL. and Freemont, PS. 1996. Curr Opin Struct Biol. 6: 395-401. PMID: 8804826
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF219 Antibody (A-4) | sc-514812 | 200 µg/ml | $322.00 | |||
RNF219 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-531225 | 200 µg Ab; 10 µg BP | $361.00 | |||
RNF219 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-524851 | 200 µg Ab, 40 µg BP | $361.00 | |||
RNF219 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-544441 | 200 µg Ab; 20 µg BP | $361.00 | |||
RNF219 Antibody (A-4) AC | sc-514812 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RNF219 Antibody (A-4) HRP | sc-514812 HRP | 200 µg/ml | $322.00 | |||
RNF219 Antibody (A-4) FITC | sc-514812 FITC | 200 µg/ml | $336.00 | |||
RNF219 Antibody (A-4) PE | sc-514812 PE | 200 µg/ml | $349.00 | |||
RNF219 Antibody (A-4) Alexa Fluor® 488 | sc-514812 AF488 | 200 µg/ml | $364.00 | |||
RNF219 Antibody (A-4) Alexa Fluor® 546 | sc-514812 AF546 | 200 µg/ml | $364.00 | |||
RNF219 Antibody (A-4) Alexa Fluor® 594 | sc-514812 AF594 | 200 µg/ml | $364.00 | |||
RNF219 Antibody (A-4) Alexa Fluor® 647 | sc-514812 AF647 | 200 µg/ml | $364.00 | |||
RNF219 Antibody (A-4) Alexa Fluor® 680 | sc-514812 AF680 | 200 µg/ml | $364.00 | |||
RNF219 Antibody (A-4) Alexa Fluor® 790 | sc-514812 AF790 | 200 µg/ml | $364.00 |