RNase HII-A Antibody (G-10): sc-515475
- RNase HII-A Antibody (G-10) is a mouse monoclonal IgG1 κ RNase HII-A antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-299 representing full length RNase HII-A of human origin
- RNase HII-A Antibody (G-10) is recommended for detection of RNase HII-A of human origin by WB, IP, IF and ELISA
- Anti-RNase HII-A Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RNase HII-A Antibody (G-10) for WB applications. These reagents are now offered in bundles with RNase HII-A Antibody (G-10) (see ordering information below).
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RNase HII-A Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNase HII-A of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RNase HII-A antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNase HII-A, also known as RNASEH2A, RNASEHI, AGS4, or RNHIA, is a crucial 299 amino acid catalytic subunit of the RNase HII complex, which plays a vital role in DNA replication by degrading RNA within RNA:DNA duplexes. RNase HII-A is primarily localized in the nucleus, where RNase HII-A is responsible for removing Okazaki fragment RNA primers from the lagging strand during DNA synthesis, ensuring the integrity and continuity of the newly synthesized DNA. RNase HII-A catalyzes the endonucleolytic cleavage of RNA to a 5′-phosphomonoester and requires magnesium or manganese as cofactors for activity. Mutations in the gene encoding RNase HII-A are linked to Aicardi-Goutieres syndrome type 4 (AGS4), a severe autosomal recessive encephalopathy that leads to significant neurological impairments, including cerebral atrophy and chronic cerebrospinal fluid lymphocytosis, often resulting in early childhood mortality. RNase HII-A′s interaction with other proteins and essential function in DNA replication highlight its importance in cellular processes and disease mechanisms, making RNase HII-A monoclonal antibody (G-10) a valuable tool for researchers studying these critical pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNase HII-A Antibody (G-10) References:
- The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides. | ten Asbroek, AL., et al. 2002. Eur J Biochem. 269: 583-92. PMID: 11856317
- RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. | Jeong, HS., et al. 2004. Nucleic Acids Res. 32: 407-14. PMID: 14734815
- Destabilization of tetranucleotide repeats in Haemophilus influenzae mutants lacking RnaseHI or the Klenow domain of PolI. | Bayliss, CD., et al. 2005. Nucleic Acids Res. 33: 400-8. PMID: 15653640
- Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutières syndrome and mimic congenital viral brain infection. | Crow, YJ., et al. 2006. Nat Genet. 38: 910-6. PMID: 16845400
- Clinical and molecular phenotype of Aicardi-Goutieres syndrome. | Rice, G., et al. 2007. Am J Hum Genet. 81: 713-25. PMID: 17846997
- Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII. | Frank, P., et al. 1998. Proc Natl Acad Sci U S A. 95: 12872-7. PMID: 9789007
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNase HII-A Antibody (G-10) | sc-515475 | 200 µg/ml | $316.00 | |||
RNase HII-A Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-531385 | 200 µg Ab; 10 µg BP | $354.00 | |||
RNase HII-A Antibody (G-10): m-IgGκ BP-HRP Bundle | sc-525062 | 200 µg Ab, 40 µg BP | $354.00 | |||
RNase HII-A Antibody (G-10): m-IgG1 BP-HRP Bundle | sc-544519 | 200 µg Ab; 20 µg BP | $354.00 | |||
RNase HII-A Antibody (G-10) AC | sc-515475 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RNase HII-A Antibody (G-10) HRP | sc-515475 HRP | 200 µg/ml | $316.00 | |||
RNase HII-A Antibody (G-10) FITC | sc-515475 FITC | 200 µg/ml | $330.00 | |||
RNase HII-A Antibody (G-10) PE | sc-515475 PE | 200 µg/ml | $343.00 | |||
RNase HII-A Antibody (G-10) Alexa Fluor® 488 | sc-515475 AF488 | 200 µg/ml | $357.00 | |||
RNase HII-A Antibody (G-10) Alexa Fluor® 546 | sc-515475 AF546 | 200 µg/ml | $357.00 | |||
RNase HII-A Antibody (G-10) Alexa Fluor® 594 | sc-515475 AF594 | 200 µg/ml | $357.00 | |||
RNase HII-A Antibody (G-10) Alexa Fluor® 647 | sc-515475 AF647 | 200 µg/ml | $357.00 | |||
RNase HII-A Antibody (G-10) Alexa Fluor® 680 | sc-515475 AF680 | 200 µg/ml | $357.00 | |||
RNase HII-A Antibody (G-10) Alexa Fluor® 790 | sc-515475 AF790 | 200 µg/ml | $357.00 |