RNase H1 Antibody (S-34): sc-101114
- RNase H1 Antibody (S-34) is a mouse monoclonal IgG2a κ provided at 100 µg/ml
- raised against recombinant RNase H1 of human origin
- recommended for detection of RNase H1 of mouse, rat and human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RNase H1 Antibody (S-34). This work is in progress.
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RNase H1 Antibody (S-34) is a mouse monoclonal IgG2a antibody that detects RNase H1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). RNase H1 is a crucial cytoplasmic endonuclease that specifically degrades the RNA strand of RNA-DNA hybrids, producing 5′-phosphomonoester products, which is vital for maintaining genomic integrity and facilitating proper DNA replication and repair processes. The activity of RNase H1 can be inhibited by agents such as manganese ions and N-ethylmaleimide, highlighting the importance of metal ion regulation in its function. Notably, RNase H1 is consistently expressed across various human tissues, suggesting its role as a housekeeping protein essential for cellular homeostasis. The gene is located on chromosome 2p25.3, with pseudogenes identified on chromosomes 17p11.2 and 1q, indicating a complex evolutionary history and potential regulatory mechanisms that may influence its expression. Anti-RNase H1 antibody (S-34) is an invaluable tool for researchers studying RNA metabolism and the molecular mechanisms underlying nucleic acid interactions.
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RNase H1 Antibody (S-34) References:
- The Jekyll and Hyde character of RNase H1 and its multiple roles in mitochondrial DNA metabolism. | Holt, IJ. 2019. DNA Repair (Amst). 84: 102630. PMID: 31178343
- RNase H1 and H2 Are Differentially Regulated to Process RNA-DNA Hybrids. | Lockhart, A., et al. 2019. Cell Rep. 29: 2890-2900.e5. PMID: 31775053
- Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. | Crossley, MP., et al. 2021. J Cell Biol. 220: PMID: 34232287
- RNA modifications can affect RNase H1-mediated PS-ASO activity. | Doxtader Lacy, KA., et al. 2022. Mol Ther Nucleic Acids. 28: 814-828. PMID: 35664704
- RNase H1 Hybrid-Binding Domain-Based Tools for Cellular Biology Studies of DNA-RNA Hybrids in Mammalian Cells. | Silva, S., et al. 2022. Methods Mol Biol. 2528: 115-125. PMID: 35704188
- NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides. | Zhang, L., et al. 2022. Nucleic Acid Ther. 32: 280-299. PMID: 35852833
- Mammalian RNase H1 directs RNA primer formation for mtDNA replication initiation and is also necessary for mtDNA replication completion. | Misic, J., et al. 2022. Nucleic Acids Res. 50: 8749-8766. PMID: 35947649
- RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis. | Liu, C., et al. 2023. Nucleic Acids Res. 51: 7357-7375. PMID: 37378420
- The ARID1A-METTL3-m6A axis ensures effective RNase H1-mediated resolution of R-loops and genome stability. | Zhang, J., et al. 2024. Cell Rep. 43: 113779. PMID: 38358891
- RPA transforms RNase H1 to a bidirectional exoribonuclease for processive RNA-DNA hybrid cleavage. | Li, Y., et al. 2024. Nat Commun. 15: 7464. PMID: 39198528
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNase H1 Antibody (S-34) | sc-101114 | 100 µg/ml | $339.00 |