RNase H1 Antibody (H-9): sc-365267
- RNase H1 Antibody (H-9) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 253-278 at the C-terminus of RNase H1 of human origin
- recommended for detection of RNase H1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for RNase H1 Antibody (H-9) for WB applications. This reagent is now offered in a bundle with RNase H1 Antibody (H-9) (see ordering information below).
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RNase H1 Antibody (H-9) is a mouse monoclonal IgG3 antibody that detects RNase H1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNase H1 is a crucial cytoplasmic endonuclease that plays a vital role in the degradation of RNA within RNA-DNA hybrids, producing 5′-phosphomonoester products that are essential for maintaining genomic integrity and facilitating proper DNA replication and repair processes. The activity of RNase H1 can be inhibited by agents such as manganese ions and N-ethylmaleimide, highlighting the importance of metal ion regulation in its function. Notably, RNase H1 is consistently expressed across various human tissues, suggesting its role as a housekeeping protein, and is located on chromosome 2p25.3, with pseudogenes identified on chromosomes 17p11.2 and 1q. RNase H1 (H-9) antibody is an invaluable tool for researchers studying RNA metabolism and the molecular mechanisms underlying cellular responses to DNA damage.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNase H1 Antibody (H-9) References:
- Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1. | Nguyen, TN., et al. 2012. Protein Sci. 21: 553-61. PMID: 22389131
- Human RNase H1 is associated with protein P32 and is involved in mitochondrial pre-rRNA processing. | Wu, H., et al. 2013. PLoS One. 8: e71006. PMID: 23990920
- RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus. | Liang, XH., et al. 2017. Mol Ther. 25: 2075-2092. PMID: 28663102
- A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL. | Al-Behadili, A., et al. 2018. Nucleic Acids Res. 46: 9471-9483. PMID: 30102370
- RNase H1 directs origin-specific initiation of DNA replication in human mitochondria. | Posse, V., et al. 2019. PLoS Genet. 15: e1007781. PMID: 30605451
- RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. | Reyes, A., et al. 2019. Front Genet. 10: 1393. PMID: 32082360
- Evolutionary History and Activity of RNase H1-Like Proteins in Arabidopsis thaliana. | Kuciński, J., et al. 2020. Plant Cell Physiol. 61: 1107-1119. PMID: 32191307
- Binding of phosphorothioate oligonucleotides with RNase H1 can cause conformational changes in the protein and alter the interactions of RNase H1 with other proteins. | Zhang, L., et al. 2021. Nucleic Acids Res. 49: 2721-2739. PMID: 33577678
- Retron reverse transcriptase termination and phage defense are dependent on host RNase H1. | Palka, C., et al. 2022. Nucleic Acids Res. 50: 3490-3504. PMID: 35293583
- The CHCHD2/Sirt1 corepressors involve in G9a-mediated regulation of RNase H1 expression to control R-loop. | Li, L., et al. 2023. Cell Insight. 2: 100112. PMID: 37388553
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNase H1 Antibody (H-9) | sc-365267 | 200 µg/ml | $322.00 | |||
RNase H1 Antibody (H-9): m-IgG3 BP-HRP Bundle | sc-550422 | 200 µg Ab; 40 µg BP | $361.00 | |||
RNase H1 (H-9) Neutralizing Peptide | sc-365267 P | 100 µg/0.5 ml | $69.00 |