RIP140 Antibody (F-2): sc-518071
- RIP140 Antibody (F-2) is a mouse monoclonal IgG2a κ RIP140 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1125-1152 at the C-terminus of RIP140 of human origin
- RIP140 Antibody (F-2) is recommended for detection of RIP140 of human origin by WB, IP, IF and ELISA
- Anti-RIP140 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RIP140 Antibody (F-2) for WB applications. These reagents are now offered in bundles with RIP140 Antibody (F-2) (see ordering information below).
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RIP140 Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects RIP140 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RIP140 antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RIP140 protein, also known as nuclear receptor interacting protein 1, plays a crucial role in the regulation of gene expression as a transcriptional coactivator and corepressor. RIP140 monoclonal antibody (F-2) interacts with the activation function 2 (AF2) domain of nuclear receptors, which are essential for mediating the effects of steroid hormones, thyroid hormones, and retinoic acids. This interaction is vital for the precise control of transcriptional responses to hormonal signals, influencing various physiological processes such as metabolism, development, and reproduction. RIP140 (F-2) antibody is known to interact with several key proteins, including GRIP1, SRC-1, RAC3, and p/CIP, which further underscores its importance in the complex network of transcriptional regulation. Using RIP140 (F-2) monoclonal antibody in your research can provide valuable insights into the mechanisms of hormone action and the role of transcriptional coactivators and corepressors in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RIP140 Antibody (F-2) References:
- Negative regulation of hormone signaling by RIP140. | Augereau, P., et al. 2006. J Steroid Biochem Mol Biol. 102: 51-9. PMID: 17056252
- Post-translational modifications of nuclear co-repressor RIP140: a therapeutic target for metabolic diseases. | Mostaqul Huq, MD., et al. 2008. Curr Med Chem. 15: 386-92. PMID: 18288993
- Distinct functions for RIP140 in development, inflammation, and metabolism. | Nautiyal, J., et al. 2013. Trends Endocrinol Metab. 24: 451-9. PMID: 23742741
- RIP140 increases APC expression and controls intestinal homeostasis and tumorigenesis. | Lapierre, M., et al. 2014. J Clin Invest. 124: 1899-913. PMID: 24667635
- The emerging role of the transcriptional coregulator RIP140 in solid tumors. | Lapierre, M., et al. 2015. Biochim Biophys Acta. 1856: 144-50. PMID: 26116758
- Nuclear receptor co-repressor RIP140 regulates diurnal expression of cytochrome P450 2b10 in mouse liver. | Zhao, M., et al. 2020. Xenobiotica. 50: 1139-1148. PMID: 32238093
- Cognitive impairments in adult mice with RIP140 overexpression in neural stem cells. | Wang, X., et al. 2020. Behav Brain Res. 393: 112777. PMID: 32579978
- RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer. | Gleizes, A., et al. 2021. Cancers (Basel). 13: PMID: 34206767
- RIP140-Mediated NF-κB Inflammatory Pathway Promotes Metabolic Dysregulation in Retinal Pigment Epithelium Cells. | Guo, Z., et al. 2022. Curr Issues Mol Biol. 44: 5788-5801. PMID: 36421677
- RIP140 regulates transcription factor HES1 oscillatory expression and mitogenic activity in colon cancer cells. | Sfeir, N., et al. 2024. Mol Oncol. 18: 1510-1530. PMID: 38459621
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIP140 Antibody (F-2) | sc-518071 | 200 µg/ml | $316.00 | |||
RIP140 Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-526445 | 200 µg Ab; 10 µg BP | $354.00 | |||
RIP140 Antibody (F-2): m-IgG2a BP-HRP Bundle | sc-546645 | 200 µg Ab; 10 µg BP | $354.00 | |||
RIP140 Antibody (F-2) AC | sc-518071 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RIP140 Antibody (F-2) HRP | sc-518071 HRP | 200 µg/ml | $316.00 | |||
RIP140 Antibody (F-2) FITC | sc-518071 FITC | 200 µg/ml | $330.00 | |||
RIP140 Antibody (F-2) PE | sc-518071 PE | 200 µg/ml | $343.00 | |||
RIP140 Antibody (F-2) Alexa Fluor® 488 | sc-518071 AF488 | 200 µg/ml | $357.00 | |||
RIP140 Antibody (F-2) Alexa Fluor® 546 | sc-518071 AF546 | 200 µg/ml | $357.00 | |||
RIP140 Antibody (F-2) Alexa Fluor® 594 | sc-518071 AF594 | 200 µg/ml | $357.00 | |||
RIP140 Antibody (F-2) Alexa Fluor® 647 | sc-518071 AF647 | 200 µg/ml | $357.00 | |||
RIP140 Antibody (F-2) Alexa Fluor® 680 | sc-518071 AF680 | 200 µg/ml | $357.00 | |||
RIP140 Antibody (F-2) Alexa Fluor® 790 | sc-518071 AF790 | 200 µg/ml | $357.00 |