RICK Antibody (A-10): sc-166765

RICK Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects RICK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RICK antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RICK, also known as receptor interacting serine/threonine kinase 2 (RIPK2) or CARDIAK, plays a crucial role in regulating apoptosis and activating nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). RICK protein is primarily located in the cytoplasm, where RICK interacts with various signaling complexes essential for mediating cellular responses to stress and inflammation. RICK′s localization allows rapid response to signals from tumor necrosis factor receptors (TNFR) and other apoptotic pathways, facilitating recruitment of downstream signaling molecules. RICK′s presence in these complexes is vital for transduction of apoptotic signals, and RICK dysregulation can lead to pathological conditions, including cancer and autoimmune diseases. Understanding RICK′s cellular dynamics and interactions provides insights into RICK′s role in immune responses and potential therapeutic targets for modulating apoptosis in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RICK Antibody (A-10) References:

1. SNO-MLP (S-Nitrosylation of Muscle LIM Protein) Facilitates Myocardial Hypertrophy Through TLR3 (Toll-Like Receptor 3)-Mediated RIP3 (Receptor-Interacting Protein Kinase 3) and NLRP3 (NOD-Like Receptor Pyrin Domain Containing 3) Inflammasome Activation.  |  Tang, X., et al. 2020. Circulation. 141: 984-1000. PMID: 31902237
2. Novel Roles of Necroptosis Mediator Receptor-Interacting Protein Kinase 3 in Kidney Injury.  |  Uni, R. and Choi, ME. 2022. Nephron. 146: 259-263. PMID: 34284405
3. Caspase-8 Blocks Receptor-Interacting Protein Kinase-1 Kinase-Independent Necroptosis during Embryogenesis.  |  Zhang, H., et al. 2022. Immunohorizons. 6: 465-475. PMID: 35858757
4. Investigating the role of receptor interacting protein kinase 3 in venous thrombosis.  |  DeRoo, E., et al. 2022. JVS Vasc Sci. 3: 365-378. PMID: 36568281
5. The pathophysiological role of receptor-interacting protein kinase 3 in cardiovascular disease.  |  Zhang, J., et al. 2023. Biomed Pharmacother. 165: 114696. PMID: 37329707
6. Receptor-interacting protein kinase 2 (RIPK2) profoundly contributes to post-stroke neuroinflammation and behavioral deficits with microglia as unique perpetrators.  |  Larochelle, J., et al. 2023. J Neuroinflammation. 20: 221. PMID: 37777791
7. Receptor interacting protein kinase-3 mediates both myopathy and cardiomyopathy in preclinical animal models of Duchenne muscular dystrophy.  |  Bencze, M., et al. 2023. J Cachexia Sarcopenia Muscle. 14: 2520-2531. PMID: 37909859
8. Elevated peripheral levels of receptor-interacting protein kinase 1 (RIPK1) and IL-8 as biomarkers of human amyotrophic lateral sclerosis.  |  Wei, J., et al. 2023. Signal Transduct Target Ther. 8: 451. PMID: 38086800
9. Bladder-cancer-derived exosomal circRNA_0013936 promotes suppressive immunity by up-regulating fatty acid transporter protein 2 and down-regulating receptor-interacting protein kinase 3 in PMN-MDSCs.  |  Shi, X., et al. 2024. Mol Cancer. 23: 52. PMID: 38461272
10. Pharmacological inhibition of receptor-interacting protein kinase 2 (RIPK2) elicits neuroprotective effects following experimental ischemic stroke.  |  Larochelle, J., et al. 2024. Exp Neurol. 377: 114812. PMID: 38729551

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RICK Antibody (A-10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-RICK Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RICK Antibody (A-10) Specifications

Alternate Product Name: RIPK2

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-540.

Epitope Target: RICK

Epitope Species: human

Product Reactivity/Detection: RICK

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for RICK Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred RICK antibody is RICK Antibody (25): sc-136059.