Ribosomal Protein S6 Antibody (C-8): sc-74459

Ribosomal Protein S6 Antibody (C-8) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect Ribosomal Protein S6 (RPS6), also known as S6, a crucial component of the 40S ribosomal subunit involved in protein synthesis. anti-RPS6 antibody (C-8) targets the N-terminal epitope comprising amino acids 1-249 of human RPS6, ensuring precise recognition and binding. RPS6 plays a vital role in the regulation of cell growth and proliferation by serving as a major substrate for S6 kinases, which phosphorylate it in response to various extracellular signals. This phosphorylation is essential for the control of protein translation and, consequently, cellular function and metabolism. RPS6 monoclonal antibody (C-8) demonstrates broad species reactivity, effectively recognizing RPS6 from mouse, rat, and human origins across multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Additionally, anti-RPS6 antibody (C-8) is available in both non-conjugated form and various conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for diverse experimental needs. The high conservation of RPS6 across eukaryotic species underscores its fundamental role in ribosomal function and cellular biology, making RPS6 (C-8) antibody an invaluable tool for research in areas such as cell signaling, growth regulation, and disease mechanisms.

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Ribosomal Protein S6 Antibody (C-8) References:

1. Ribosomal protein S6 cDNA from two Aedes mosquitoes encodes a carboxyl-terminal extension that resembles histone H1 proteins.  |  Hernandez, VP. and Fallon, AM. 1999. Genetica. 106: 263-7. PMID: 10897799
2. The mapping of seven intron-containing ribosomal protein genes shows they are unlinked in the human genome.  |  Feo, S., et al. 1992. Genomics. 13: 201-7. PMID: 1577483
3. Regulation and function of ribosomal protein S6 kinase (S6K) within mTOR signalling networks.  |  Magnuson, B., et al. 2012. Biochem J. 441: 1-21. PMID: 22168436
4. Ribosomal Protein S6 Phosphorylation: Four Decades of Research.  |  Meyuhas, O. 2015. Int Rev Cell Mol Biol. 320: 41-73. PMID: 26614871
5. Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe.  |  Gross, T., et al. 1988. Curr Genet. 13: 57-63. PMID: 2834104
6. Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6.  |  Lott, JB. and Mackie, GA. 1988. Gene. 65: 31-9. PMID: 2840355
7. Phosphoproteomic Analysis of Neonatal Regenerative Myocardium Revealed Important Roles of Checkpoint Kinase 1 via Activating Mammalian Target of Rapamycin C1/Ribosomal Protein S6 Kinase b-1 Pathway.  |  Fan, Y., et al. 2020. Circulation. 141: 1554-1569. PMID: 32098494
8. The primary structure of the human ribosomal protein S6 derived from a cloned cDNA.  |  Heinze, H., et al. 1988. J Biol Chem. 263: 4139-44. PMID: 3279029
9. The Role of Ribosomal Protein S6 Kinases in Plant Homeostasis.  |  Obomighie, I., et al. 2021. Front Mol Biosci. 8: 636560. PMID: 33778006
10. Blocking ribosomal protein S6 phosphorylation inhibits podocyte hypertrophy and focal segmental glomerulosclerosis.  |  Li, F., et al. 2022. Kidney Int. 102: 121-135. PMID: 35483522
11. Hepatic ribosomal protein S6 (Rps6) insufficiency results in failed bile duct development and loss of hepatocyte viability; a ribosomopathy-like phenotype that is partially p53-dependent.  |  Comerford, SA., et al. 2023. PLoS Genet. 19: e1010595. PMID: 36656901
12. The ribosomal protein S6 kinase alpha-1 (RPS6KA1) induces resistance to venetoclax/azacitidine in acute myeloid leukemia.  |  Weidenauer, K., et al. 2023. Leukemia. 37: 1611-1625. PMID: 37414921

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ribosomal Protein S6 Antibody (C-8) has 181 citations in a variety of scientific publications.

Primary antibodies like Anti-Ribosomal Protein S6 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ribosomal Protein S6 Antibody (C-8) Specifications

Alternate Product Name: Ribosomal Protein S6

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-249

Epitope Target: Ribosomal Protein S6

Epitope Species: human

Product Reactivity/Detection: 40S Ribosomal Protein S6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 181 citations in various scientific publications

Available Conjugates/Forms for Ribosomal Protein S6 Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin