RhoGEF p115 Antibody (C-9): sc-74565
- RhoGEF p115 Antibody (C-9) is a mouse monoclonal IgG2b κ RhoGEF p115 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 748-912 mapping at the C-terminus of RhoGEF p115 of human origin
- RhoGEF p115 Antibody (C-9) is recommended for detection of RhoGEF p115 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-RhoGEF p115 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RhoGEF p115 Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with RhoGEF p115 Antibody (C-9) (see ordering information below).
QUICK LINKS
RhoGEF p115 Antibody (C-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects RhoGEF p115 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-RhoGEF p115 antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RhoGEF p115 plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor (GEF) specifically for Rho family GTPases, which are pivotal in regulating cytoskeletal dynamics, cell migration, and growth. RhoGEF p115 is primarily located in the cytoplasm, where RhoGEF p115 interacts with various signaling pathways to facilitate the activation of Rho GTPases, thereby influencing processes such as actin cytoskeleton organization and cellular adhesion. Understanding the localization and function of RhoGEF p115 is essential, as dysregulation of Rho signaling is implicated in numerous diseases, including cancer and cardiovascular disorders. Using RhoGEF p115 monoclonal antibody (C-9) in research can provide valuable insights into the mechanisms of Rho GTPase regulation and its impact on cellular behavior.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RhoGEF p115 Antibody (C-9) References:
- Reversible translocation of p115-RhoGEF by G(12/13)-coupled receptors. | Meyer, BH., et al. 2008. J Cell Biochem. 104: 1660-70. PMID: 18320579
- Differences in Galpha12- and Galpha13-mediated plasma membrane recruitment of p115-RhoGEF. | Bhattacharyya, R., et al. 2009. Cell Signal. 21: 996-1006. PMID: 19249348
- Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF. | Chen, Z., et al. 2011. Protein Sci. 20: 107-17. PMID: 21064165
- The GTPase superfamily: a conserved switch for diverse cell functions. | Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
- Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain. | Chen, Z., et al. 2012. J Biol Chem. 287: 25490-500. PMID: 22661716
- Proteins regulating Ras and its relatives. | Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
- Expression cloning of lsc, a novel oncogene with structural similarities to the Dbl family of guanine nucleotide exchange factors. | Whitehead, IP., et al. 1996. J Biol Chem. 271: 18643-50. PMID: 8702517
- The Dbl family of oncogenes. | Cerione, RA. and Zheng, Y. 1996. Curr Opin Cell Biol. 8: 216-22. PMID: 8791419
- Identification of a novel guanine nucleotide exchange factor for the Rho GTPase. | Hart, MJ., et al. 1996. J Biol Chem. 271: 25452-8. PMID: 8810315
- Dbl family proteins. | Whitehead, IP., et al. 1997. Biochim Biophys Acta. 1332: F1-23. PMID: 9061011
- p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. | Kozasa, T., et al. 1998. Science. 280: 2109-11. PMID: 9641915
- Rho family proteins and Ras transformation: the RHOad less traveled gets congested. | Zohn, IM., et al. 1998. Oncogene. 17: 1415-38. PMID: 9779988
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RhoGEF p115 Antibody (C-9) | sc-74565 | 200 µg/ml | $316.00 | |||
RhoGEF p115 Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-528693 | 200 µg Ab; 10 µg BP | $354.00 | |||
RhoGEF p115 Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-521143 | 200 µg Ab, 40 µg BP | $354.00 | |||
RhoGEF p115 Antibody (C-9): m-IgG2b BP-HRP Bundle | sc-550067 | 200 µg Ab; 10 µg BP | $354.00 | |||
RhoGEF p115 Antibody (C-9) AC | sc-74565 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RhoGEF p115 Antibody (C-9) HRP | sc-74565 HRP | 200 µg/ml | $316.00 | |||
RhoGEF p115 Antibody (C-9) FITC | sc-74565 FITC | 200 µg/ml | $330.00 | |||
RhoGEF p115 Antibody (C-9) PE | sc-74565 PE | 200 µg/ml | $343.00 | |||
RhoGEF p115 Antibody (C-9) Alexa Fluor® 488 | sc-74565 AF488 | 200 µg/ml | $357.00 | |||
RhoGEF p115 Antibody (C-9) Alexa Fluor® 546 | sc-74565 AF546 | 200 µg/ml | $357.00 | |||
RhoGEF p115 Antibody (C-9) Alexa Fluor® 594 | sc-74565 AF594 | 200 µg/ml | $357.00 | |||
RhoGEF p115 Antibody (C-9) Alexa Fluor® 647 | sc-74565 AF647 | 200 µg/ml | $357.00 | |||
RhoGEF p115 Antibody (C-9) Alexa Fluor® 680 | sc-74565 AF680 | 200 µg/ml | $357.00 | |||
RhoGEF p115 Antibody (C-9) Alexa Fluor® 790 | sc-74565 AF790 | 200 µg/ml | $357.00 |