RGS8 Antibody (H-7): sc-390393

RGS8 Antibody (H-7) is a mouse monoclonal IgG1 antibody that detects RGS8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGS8 is a critical regulator of G protein signaling, functioning as a GTPase-activating protein that enhances the GTPase activity of G protein alpha-subunits, thus promoting their transition to an inactive GDP-bound state. RGS8 is predominantly expressed in the brain, particularly within the Purkinje cells of the cerebellum, where RGS8 plays a unique role compared to other RGS proteins by positively influencing G-protein-coupled inwardly rectifying K+ (GIRK1/2) channels. This distinctive function suggests that RGS8 not only serves as a negative regulator but also enhances the kinetic efficacy of G-proteins, which is essential for maintaining proper neuronal signaling and excitability. The NH2 terminus of RGS8 is crucial for cellular localization, underscoring the importance of spatial distribution in modulating G protein signaling pathways. Anti-RGS8 antibody (H-7) is an invaluable tool for researchers investigating the complex dynamics of G protein signaling and its implications in various physiological and pathological processes.

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RGS8 Antibody (H-7) References:

1. RGS8 protein is distributed in dendrites and cell body of cerebellar Purkinje cell.  |  Itoh, M., et al. 2001. Biochem Biophys Res Commun. 287: 223-8. PMID: 11549278
2. Alternative splicing of RGS8 gene determines inhibitory function of receptor type-specific Gq signaling.  |  Saitoh, O., et al. 2002. Proc Natl Acad Sci U S A. 99: 10138-43. PMID: 12110731
3. RGS8 expression in developing cerebellar Purkinje cells.  |  Saitoh, O. and Odagiri, M. 2003. Biochem Biophys Res Commun. 309: 836-42. PMID: 13679049
4. Spinophilin inhibits the binding of RGS8 to M1-mAChR but enhances the regulatory function of RGS8.  |  Fujii, S., et al. 2008. Biochem Biophys Res Commun. 377: 200-4. PMID: 18834863
5. Ataxin-2 regulates RGS8 translation in a new BAC-SCA2 transgenic mouse model.  |  Dansithong, W., et al. 2015. PLoS Genet. 11: e1005182. PMID: 25902068
6. Depression-resistant Phenotype in Mice Overexpressing Regulator of G Protein Signaling 8 (RGS8).  |  Kobayashi, Y., et al. 2018. Neuroscience. 383: 160-169. PMID: 29758252
7. Modulation of Increased mGluR1 Signaling by RGS8 Protects Purkinje Cells From Dendritic Reduction and Could Be a Common Mechanism in Diverse Forms of Spinocerebellar Ataxia.  |  Wu, QW. and Kapfhammer, JP. 2020. Front Cell Dev Biol. 8: 569889. PMID: 33553137
8. Autoantibodies Against the Purkinje Cell Protein RGS8 in Paraneoplastic Cerebellar Syndrome.  |  Miske, R., et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8: PMID: 33782191
9. CRISPR-Cas13-Mediated Knockdown of Regulator of G-Protein Signaling 8 (RGS8) Does Not Affect Purkinje Cell Dendritic Development.  |  Wu, QW. and Kapfhammer, JP. 2022. Front Cell Dev Biol. 10: 854273. PMID: 35712654
10. Anti-RGS8 paraneoplastic cerebellar ataxia is preferentially associated with a particular subtype of Hodgkin's lymphoma.  |  Peter, E., et al. 2024. J Neurol. 271: 6839-6846. PMID: 39207522

The preferred RGS8 antibody is RGS8 Antibody (F-3): sc-398949.