RGS8 Antibody (F-3): sc-398949

RGS8 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RGS8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGS8 Antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RGS8 protein, consisting of 180 amino acids, plays a crucial role in the regulation of G protein signaling, particularly in the central nervous system where RGS8 is predominantly expressed in the Purkinje cells of the cerebellum. Unlike most RGS proteins that primarily act as negative regulators, RGS8 uniquely enhances the activity of G-protein-coupled inwardly rectifying potassium channels (GIRK1/2), thereby contributing to the modulation of neuronal excitability and synaptic transmission. This dual regulatory function of RGS8 is significant as RGS8 suggests a complex role in fine-tuning G protein signaling pathways, which are essential for various physiological processes, including mood regulation and motor control. The NH2 terminus of RGS8 is critical for localization within the cell, ensuring proper positioning to effectively interact with target proteins and modulate their activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RGS8 Antibody (F-3) References:

1. RGS7 and RGS8 differentially accelerate G protein-mediated modulation of K+ currents.  |  Saitoh, O., et al. 1999. J Biol Chem. 274: 9899-904. PMID: 10092682
2. Regulator of G protein signaling 8 (RGS8) requires its NH2 terminus for subcellular localization and acute desensitization of G protein-gated K+ channels.  |  Saitoh, O., et al. 2001. J Biol Chem. 276: 5052-8. PMID: 11087736
3. Alternative splicing of RGS8 gene determines inhibitory function of receptor type-specific Gq signaling.  |  Saitoh, O., et al. 2002. Proc Natl Acad Sci U S A. 99: 10138-43. PMID: 12110731
4. Distribution of regulator of G protein signaling 8 (RGS8) protein in the cerebellum.  |  Saitoh, O., et al. 2003. Cerebellum. 2: 154-60. PMID: 12880183
5. Biochemical and electrophysiological analyses of RGS8 function.  |  Saitoh, O. and Yoshihiro, K. 2004. Methods Enzymol. 390: 129-48. PMID: 15488175
6. Participation of RGS8 in the ternary complex of agonist, receptor and G-protein.  |  Benians, A., et al. 2004. Biochem Soc Trans. 32: 1045-7. PMID: 15506959
7. Regulators of G-protein signaling form a quaternary complex with the agonist, receptor, and G-protein. A novel explanation for the acceleration of signaling activation kinetics.  |  Benians, A., et al. 2005. J Biol Chem. 280: 13383-94. PMID: 15677457
8. Expression of regulator of G protein signalling proteins in natural killer cells, and their modulation by Ly49A and Ly49D.  |  Kveberg, L., et al. 2005. Immunology. 115: 358-65. PMID: 15946253
9. Alternative splicing of RGS8 gene changes the binding property to the M1 muscarinic receptor to confer receptor type-specific Gq regulation.  |  Itoh, M., et al. 2006. J Neurochem. 99: 1505-16. PMID: 17064349
10. Regulation of melanin-concentrating hormone receptor 1 signaling by RGS8 with the receptor third intracellular loop.  |  Miyamoto-Matsubara, M., et al. 2008. Cell Signal. 20: 2084-94. PMID: 18760349
11. Effects of spinophilin on the function of RGS8 regulating signals from M2 and M3-mAChRs.  |  Kurogi, M., et al. 2009. Neuroreport. 20: 1134-9. PMID: 19609226
12. RGS8 accelerates G-protein-mediated modulation of K+ currents.  |  Saitoh, O., et al. 1997. Nature. 390: 525-9. PMID: 9394004

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RGS8 Antibody (F-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RGS8 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RGS8 Antibody (F-3) Specifications

Alternate Product Name: RGS8

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 127-146

Epitope Target: RGS8

Epitope Species: human

Product Reactivity/Detection: RGS8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RGS8 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin