RGS8 Antibody (C-5): sc-377354
- RGS8 Antibody (C-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-65 mapping at the N-terminus of RGS8 of human origin
- recommended for detection of RGS8 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RGS8 Antibody (C-5). This work is in progress.
RGS8 Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects RGS8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGS8 is a critical regulator of G protein signaling, functioning as a GTPase-activating protein that enhances the GTPase activity of G protein alpha-subunits, thus promoting their transition to an inactive GDP-bound state. Notably, RGS8 is primarily expressed in the brain, particularly within the Purkinje cells of the cerebellum, where RGS8 plays a unique role compared to other RGS proteins by positively influencing G-protein-coupled inwardly rectifying potassium channels (GIRK1/2). This distinctive function suggests that RGS8 not only serves as a negative regulator of G protein signaling but also enhances the kinetic efficacy of G proteins, which is essential for proper neuronal signaling and function. The NH2 terminus of RGS8 is crucial for cellular localization, underscoring the importance of spatial distribution in modulating signaling pathways. Anti-RGS8 antibody (C-5) is an invaluable tool for researchers investigating the complex roles of RGS proteins in cellular signaling and their implications in neurological functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RGS8 Antibody (C-5) References:
- RGS8 protein is distributed in dendrites and cell body of cerebellar Purkinje cell. | Itoh, M., et al. 2001. Biochem Biophys Res Commun. 287: 223-8. PMID: 11549278
- Alternative splicing of RGS8 gene determines inhibitory function of receptor type-specific Gq signaling. | Saitoh, O., et al. 2002. Proc Natl Acad Sci U S A. 99: 10138-43. PMID: 12110731
- RGS8 expression in developing cerebellar Purkinje cells. | Saitoh, O. and Odagiri, M. 2003. Biochem Biophys Res Commun. 309: 836-42. PMID: 13679049
- Generation of RGS8 null mutant mice by Cre/loxP system. | Kuwata, H., et al. 2008. Kobe J Med Sci. 53: 275-81. PMID: 18762722
- Ataxin-2 regulates RGS8 translation in a new BAC-SCA2 transgenic mouse model. | Dansithong, W., et al. 2015. PLoS Genet. 11: e1005182. PMID: 25902068
- Depression-resistant Phenotype in Mice Overexpressing Regulator of G Protein Signaling 8 (RGS8). | Kobayashi, Y., et al. 2018. Neuroscience. 383: 160-169. PMID: 29758252
- Modulation of Increased mGluR1 Signaling by RGS8 Protects Purkinje Cells From Dendritic Reduction and Could Be a Common Mechanism in Diverse Forms of Spinocerebellar Ataxia. | Wu, QW. and Kapfhammer, JP. 2020. Front Cell Dev Biol. 8: 569889. PMID: 33553137
- Autoantibodies Against the Purkinje Cell Protein RGS8 in Paraneoplastic Cerebellar Syndrome. | Miske, R., et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8: PMID: 33782191
- CRISPR-Cas13-Mediated Knockdown of Regulator of G-Protein Signaling 8 (RGS8) Does Not Affect Purkinje Cell Dendritic Development. | Wu, QW. and Kapfhammer, JP. 2022. Front Cell Dev Biol. 10: 854273. PMID: 35712654
- Anti-RGS8 paraneoplastic cerebellar ataxia is preferentially associated with a particular subtype of Hodgkin's lymphoma. | Peter, E., et al. 2024. J Neurol. 271: 6839-6846. PMID: 39207522
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGS8 Antibody (C-5) | sc-377354 | 200 µg/ml | $322.00 |