RFX1 Antibody (H-2): sc-376041

RFX1 Antibody (H-2) is a mouse monoclonal IgM antibody that detects RFX1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFX1 is a crucial member of the regulatory factor X (RFX) family, which plays a significant role in the transcriptional regulation of major histocompatibility complex (MHC) class II genes. RFX1 is primarily located in the nucleus, where RFX1 forms a complex with other RFX proteins, such as RFX2 and RFX3, to bind to specific DNA sequences in the promoters of target genes. This nuclear localization is vital for RFX1 function, as nuclear positioning allows RFX1 to interact with the transcriptional machinery and modulate gene expression in response to various stimuli, including interferon-gamma. RFX1′s ability to activate MHC class II genes is particularly important for the immune response, as activation enhances the presentation of antigens to T cells, thereby facilitating adaptive immunity. Anti-RFX1 antibody (H-2) is an essential tool for researchers studying immune regulation and the molecular mechanisms underlying MHC class II gene expression.

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RFX1 Antibody (H-2) References:

1. Induction of Myc-intron-binding polypeptides MIBP1 and RFX1 during retinoic acid-mediated differentiation of haemopoietic cells.  |  Zajac-Kaye, M., et al. 2000. Biochem J. 345 Pt 3: 535-41. PMID: 10642512
2. Regulation of FGF1 gene promoter through transcription factor RFX1.  |  Hsu, YC., et al. 2010. J Biol Chem. 285: 13885-95. PMID: 20189986
3. Epigenetics and SLE: RFX1 downregulation causes CD11a and CD70 overexpression by altering epigenetic modifications in lupus CD4+ T cells.  |  Zhao, M., et al. 2010. J Autoimmun. 35: 58-69. PMID: 20223637
4. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells.  |  Su, JC., et al. 2014. Oncotarget. 5: 4909-19. PMID: 24952874
5. RFX1 and RFX3 Transcription Factors Interact with the D Sequence of Adeno-Associated Virus Inverted Terminal Repeat and Regulate AAV Transduction.  |  Julien, L., et al. 2018. Sci Rep. 8: 210. PMID: 29317724
6. ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA.  |  Chen, YC., et al. 2018. Cell Death Dis. 9: 437. PMID: 29740017
7. Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma.  |  Ma, T., et al. 2020. Front Genet. 11: 607636. PMID: 33519911
8. RBM39 promotes hepatocarcinogenesis by regulating RFX1's alternative splicing and subsequent activation of integrin signaling pathway.  |  Zhang, B., et al. 2025. Oncogene.. PMID: 40033026
9. Interaction of transcription factors RFX1 and MIBP1 with the gamma motif of the negative regulatory element of the hepatitis B virus core promoter.  |  Buckwold, VE., et al. 1997. Virology. 227: 515-8. PMID: 9018153
10. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes.  |  Katan-Khaykovich, Y. and Shaul, Y. 1998. J Biol Chem. 273: 24504-12. PMID: 9733744

The preferred RFX1 antibody is RFX1 Antibody (F-6): sc-374270.