RFX1 Antibody (F-6): sc-374270

RFX1 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects RFX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFX1 Antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RFX1 protein plays a crucial role in gene expression regulation, particularly in immune response, as a key component of the regulatory factor X (RFX) complex that binds to specific DNA sequences in major histocompatibility complex (MHC) class II gene promoters. This binding enables transcriptional activation of these genes, which are vital for antigen presentation to T cells, influencing adaptive immune response. RFX1 protein primarily resides in the nucleus, interacting with other RFX family members, such as RFX2 and RFX3, to form a functional transcription factor complex. This complex enhances MHC class II gene expression in response to signals like interferon-gamma and regulates various viral genes, including hepatitis B virus. RFX1′s ability to form heterodimers and homodimers with other RFX proteins underscores RFX1′s importance in maintaining immune system functionality and highlights RFX1′s potential as a therapeutic target in immune-related diseases.

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RFX1 Antibody (F-6) References:

1. Orientation and positional mapping of the subunits of the multicomponent transcription factors RFX and X2BP to the major histocompatibility complex class II transcriptional enhancer.  |  Westerheide, SD. and Boss, JM. 1999. Nucleic Acids Res. 27: 1635-41. PMID: 10075994
2. Involvement of RFX1 protein in the regulation of the human proliferating cell nuclear antigen promoter.  |  Liu, M., et al. 1999. J Biol Chem. 274: 15433-9. PMID: 10336433
3. Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding.  |  Gajiwala, KS., et al. 2000. Nature. 403: 916-21. PMID: 10706293
4. Novel mutations within the RFX-B gene and partial rescue of MHC and related genes through exogenous class II transactivator in RFX-B-deficient cells.  |  Nagarajan, UM., et al. 2000. J Immunol. 164: 3666-74. PMID: 10725724
5. Nuclear import and DNA-binding activity of RFX1. Evidence for an autoinhibitory mechanism.  |  Katan-Khaykovich, Y. and Shaul, Y. 2001. Eur J Biochem. 268: 3108-16. PMID: 11358531
6. Functional organization of the hepatitis B virus enhancer.  |  Dikstein, R., et al. 1990. Mol Cell Biol. 10: 3683-9. PMID: 2355919
7. Transcription factor RFX1 is ubiquitinated by E3 ligase STUB1 in systemic lupus erythematosus.  |  Guo, Y., et al. 2016. Clin Immunol. 169: 1-7. PMID: 27283392
8. Transcription factor RFX1 helps control the promoter of the mouse ribosomal protein-encoding gene rpL30 by binding to its alpha element.  |  Sáfrány, G. and Perry, RP. 1993. Gene. 132: 279-83. PMID: 8224874
9. Assembly of functional regulatory complexes on MHC class II promoters in vivo.  |  Fontes, JD., et al. 1997. J Mol Biol. 270: 336-45. PMID: 9237901
10. The transcriptional activation and repression domains of RFX1, a context-dependent regulator, can mutually neutralize their activities.  |  Katan, Y., et al. 1997. Nucleic Acids Res. 25: 3621-8. PMID: 9278482
11. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes.  |  Katan-Khaykovich, Y. and Shaul, Y. 1998. J Biol Chem. 273: 24504-12. PMID: 9733744
12. A gene encoding a novel RFX-associated transactivator is mutated in the majority of MHC class II deficiency patients.  |  Masternak, K., et al. 1998. Nat Genet. 20: 273-7. PMID: 9806546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RFX1 Antibody (F-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RFX1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RFX1 Antibody (F-6) Specifications

Alternate Product Name: RFX1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 931-967

Epitope Target: RFX1

Epitope Species: human

Product Reactivity/Detection: RFX1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RFX1 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent