RFC5 Antibody (F-9): sc-376528
- RFC5 Antibody (F-9) is a mouse monoclonal IgG1 κ RFC5 antibody provided at 200 µg/ml
- raised against amino acids 161-340 mapping at the C-terminus of RFC5 of human origin
- RFC5 Antibody (F-9) is recommended for detection of RFC5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RFC5 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RFC5 Antibody (F-9) for WB applications. These reagents are now offered in bundles with RFC5 Antibody (F-9) (see ordering information below).
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RFC5 Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects RFC5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFC5 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The replication factor C (RFC) complex, which includes RFC5, plays a critical role in DNA metabolism, particularly in DNA replication, repair, and telomere maintenance. RFC5 is essential for loading proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions, a process that is ATP-dependent and crucial for the proper functioning of DNA polymerases during replication. The RFC complex is a heteropentameric assembly, and RFC5 subunit, located on chromosome 12q24.2-q24.3, interacts with other RFC subunits and proteins such as Rad24, which is vital for DNA damage checkpoint control. This interaction is particularly important in the budding yeast Saccharomyces cerevisiae, where RFC5 and RAD24 work together to transduce DNA damage signals, activating the central transducer Rad53, thereby ensuring genomic stability. Anti-RFC5 antibody (F-9) effectively detects RFC5 protein across multiple species and applications, making RFC5 (F-9) monoclonal antibody an invaluable tool for researchers studying DNA metabolism and repair mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RFC5 Antibody (F-9) References:
- A novel Rad24 checkpoint protein complex closely related to replication factor C. | Green, CM., et al. 2000. Curr Biol. 10: 39-42. PMID: 10660302
- Rfc5, in cooperation with rad24, controls DNA damage checkpoints throughout the cell cycle in Saccharomyces cerevisiae. | Naiki, T., et al. 2000. Mol Cell Biol. 20: 5888-96. PMID: 10913172
- ATP utilization by yeast replication factor C. IV. RFC ATP-binding mutants show defects in DNA replication, DNA repair, and checkpoint regulation. | Schmidt, SL., et al. 2001. J Biol Chem. 276: 34792-800. PMID: 11549622
- Characterization of the five replication factor C genes of Saccharomyces cerevisiae. | Cullmann, G., et al. 1995. Mol Cell Biol. 15: 4661-71. PMID: 7651383
- Destabilized PCNA trimers suppress defective Rfc1 proteins in vivo and in vitro. | Beckwith, WH., et al. 1998. Biochemistry. 37: 3711-22. PMID: 9521689
- The RFC2 gene, encoding the third-largest subunit of the replication factor C complex, is required for an S-phase checkpoint in Saccharomyces cerevisiae. | Noskov, VN., et al. 1998. Mol Cell Biol. 18: 4914-23. PMID: 9671499
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RFC5 Antibody (F-9) | sc-376528 | 200 µg/ml | $322.00 | |||
RFC5 Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-529896 | 200 µg Ab; 10 µg BP | $361.00 | |||
RFC5 Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-522992 | 200 µg Ab, 40 µg BP | $361.00 | |||
RFC5 Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-543753 | 200 µg Ab; 20 µg BP | $361.00 | |||
RFC5 Antibody (F-9) AC | sc-376528 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RFC5 Antibody (F-9) HRP | sc-376528 HRP | 200 µg/ml | $322.00 | |||
RFC5 Antibody (F-9) FITC | sc-376528 FITC | 200 µg/ml | $336.00 | |||
RFC5 Antibody (F-9) PE | sc-376528 PE | 200 µg/ml | $349.00 | |||
RFC5 Antibody (F-9) Alexa Fluor® 488 | sc-376528 AF488 | 200 µg/ml | $364.00 | |||
RFC5 Antibody (F-9) Alexa Fluor® 546 | sc-376528 AF546 | 200 µg/ml | $364.00 | |||
RFC5 Antibody (F-9) Alexa Fluor® 594 | sc-376528 AF594 | 200 µg/ml | $364.00 | |||
RFC5 Antibody (F-9) Alexa Fluor® 647 | sc-376528 AF647 | 200 µg/ml | $364.00 | |||
RFC5 Antibody (F-9) Alexa Fluor® 680 | sc-376528 AF680 | 200 µg/ml | $364.00 | |||
RFC5 Antibody (F-9) Alexa Fluor® 790 | sc-376528 AF790 | 200 µg/ml | $364.00 |