RFC4 Antibody (E-12): sc-28300

RFC4 Antibody (E-12) is a mouse monoclonal IgG1 antibody that detects RFC4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RFC4 is a crucial component of the replication factor C complex, which plays a vital role in DNA metabolism, including replication, repair, and telomere maintenance. RFC4 (E-12) antibody recognizes a protein that is part of a heteropentameric complex facilitating the loading of proliferating cell nuclear antigen (PCNA) onto DNA, a process essential for DNA synthesis and repair. The RFC4 subunit is involved in checkpoint regulation and is a key player in the BRCA1-associated genome surveillance complex (BASC), which monitors DNA integrity and regulates post-replication repair mechanisms. RFC4′s presence in the BASC complex underscores its importance in maintaining genomic stability, as RFC4 acts as a sensor for abnormal DNA structures. The human RFC4 gene is located on chromosome 3q27.3, and RFC4′s proper function is critical for cellular responses to DNA damage, making RFC4 (E-12) monoclonal antibody a significant tool for studies related to cancer and other genetic disorders. Anti-RFC4 antibody (E-12) is an invaluable tool for researchers investigating the intricate mechanisms of DNA metabolism and RFC4′s role in cellular processes.

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RFC4 Antibody (E-12) References:

1. Genome-wide RNAi Screening Identifies RFC4 as a Factor That Mediates Radioresistance in Colorectal Cancer by Facilitating Nonhomologous End Joining Repair.  |  Wang, XC., et al. 2019. Clin Cancer Res. 25: 4567-4579. PMID: 30979744
2. Differently Expressed Genes (DEGs) Relevant to Type 2 Diabetes Mellitus Identification and Pathway Analysis via Integrated Bioinformatics Analysis.  |  Che, X., et al. 2019. Med Sci Monit. 25: 9237-9244. PMID: 31797865
3. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness.  |  Liu, L., et al. 2021. Nat Commun. 12: 2693. PMID: 33976158
4. The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.  |  Wang, J., et al. 2021. Aging (Albany NY). 13: 21758-21777. PMID: 34520390
5. Sequential gene expression analysis of cervical malignant transformation identifies RFC4 as a novel diagnostic and prognostic biomarker.  |  Zhang, J., et al. 2022. BMC Med. 20: 437. PMID: 36352434
6. Knockdown of RFC4 inhibits the cell proliferation of nasopharyngeal carcinoma in vitro and in vivo.  |  Guan, S., et al. 2023. Front Med. 17: 132-142. PMID: 36562948
7. Identification of RFC4 as a potential biomarker for pan-cancer involving prognosis, tumour immune microenvironment and drugs.  |  Yu, L., et al. 2024. J Cell Mol Med. 28: e18478. PMID: 39031628
8. Expanding the genetic and phenotypic landscape of replication factor C complex-related disorders: RFC4 deficiency is linked to a multisystemic disorder.  |  Morimoto, M., et al. 2024. Am J Hum Genet. 111: 1970-1993. PMID: 39106866
9. RFC4 confers radioresistance of esophagus squamous cell carcinoma through regulating DNA damage response.  |  Yang, T., et al. 2025. Am J Physiol Cell Physiol. 328: C367-C380. PMID: 39672175
10. Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo.  |  You, P., et al. 2024. FEBS Open Bio.. PMID: 39673181

The preferred RFC4 antibody is RFC4 Antibody (C-9): sc-28301.