RFC4 Antibody (C-9): sc-28301

RFC4 Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects RFC4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RFC4 (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The replication factor C (RFC) complex, which includes RFC4, is crucial for DNA metabolism, playing a significant role in DNA replication, repair, and telomere maintenance. RFC4 is particularly important as RFC4 is involved in loading proliferating cell nuclear antigen (PCNA) onto DNA, a process essential for DNA synthesis and repair. This loading occurs at primer-template junctions in an ATP-dependent manner, highlighting RFC4′s role in facilitating DNA replication machinery assembly. Additionally, RFC4 is a key component of the BRCA1-associated genome surveillance complex (BASC), which functions as a sensor for abnormal DNA structures and regulates postreplication repair processes, thereby contributing to genomic stability. The human RFC4 gene is located on chromosome 3q27.3 and encodes the RFC4 subunit, underscoring RFC4′s significance in maintaining cellular integrity and preventing genomic instability.

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RFC4 Antibody (C-9) References:

1. A novel Rad24 checkpoint protein complex closely related to replication factor C.  |  Green, CM., et al. 2000. Curr Biol. 10: 39-42. PMID: 10660302
2. ATP utilization by yeast replication factor C. III. The ATP-binding domains of Rfc2, Rfc3, and Rfc4 are essential for DNA recognition and clamp loading.  |  Schmidt, SL., et al. 2001. J Biol Chem. 276: 34784-91. PMID: 11432854
3. Loss of cell cycle checkpoint control in Drosophila Rfc4 mutants.  |  Krause, SA., et al. 2001. Mol Cell Biol. 21: 5156-68. PMID: 11438670
4. ATP utilization by yeast replication factor C. IV. RFC ATP-binding mutants show defects in DNA replication, DNA repair, and checkpoint regulation.  |  Schmidt, SL., et al. 2001. J Biol Chem. 276: 34792-800. PMID: 11549622
5. Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.  |  Xiang, J., et al. 2014. J Transl Med. 12: 320. PMID: 25407051
6. CDK1, CCNB1, CDC20, BUB1, MAD2L1, MCM3, BUB1B, MCM2, and RFC4 May Be Potential Therapeutic Targets for Hepatocellular Carcinoma Using Integrated Bioinformatic Analysis.  |  Yang, WX., et al. 2019. Biomed Res Int. 2019: 1245072. PMID: 31737652
7. Replication Factor C4 in human hepatocellular carcinoma: A potent prognostic factor associated with cell proliferation.  |  Chen, P., et al. 2021. Biosci Trends. 15: 249-256. PMID: 34053971
8. Identification of DNA damage response-related genes as biomarkers for castration-resistant prostate cancer.  |  Oshima, M., et al. 2023. Sci Rep. 13: 19602. PMID: 37950047
9. Characterization of the five replication factor C genes of Saccharomyces cerevisiae.  |  Cullmann, G., et al. 1995. Mol Cell Biol. 15: 4661-71. PMID: 7651383
10. Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23.  |  Okumura, K., et al. 1995. Genomics. 25: 274-8. PMID: 7774928
11. Destabilized PCNA trimers suppress defective Rfc1 proteins in vivo and in vitro.  |  Beckwith, WH., et al. 1998. Biochemistry. 37: 3711-22. PMID: 9521689
12. The RFC2 gene, encoding the third-largest subunit of the replication factor C complex, is required for an S-phase checkpoint in Saccharomyces cerevisiae.  |  Noskov, VN., et al. 1998. Mol Cell Biol. 18: 4914-23. PMID: 9671499

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RFC4 Antibody (C-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-RFC4 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RFC4 Antibody (C-9) Specifications

Alternate Product Name: RFC4

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-363

Epitope Target: RFC4

Epitope Species: human

Product Reactivity/Detection:

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for RFC4 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin