Rent3b Antibody (D-1): sc-514564
- Rent3b Antibody (D-1) is a mouse monoclonal IgG1 κ Rent3b antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 7-26 at the N-terminus of Rent3b of human origin
- recommended for detection of Rent3b of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rent3b Antibody (D-1) for WB applications. These reagents are now offered in bundles with Rent3b Antibody (D-1) (see ordering information below).
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Rent3b (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rent3b protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Rent3b antibody (D-1) is available as the non-conjugated form. In eukaryotes, having the ability to detect and degrade transcripts lacking full coding potential is essential. Nonsense-mediated RNA decay (NMD) protects organisms by avoiding translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5′ to 3′ helicase activity. Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by separate genes. Rent3b (also known as Rent3X) is encoded by an X-linked gene and localizes primarily to the nucleus, while Rent1 and Rent2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.
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Rent3b Antibody (D-1) References:
- SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast. | Page, MF., et al. 1999. Mol Cell Biol. 19: 5943-51. PMID: 10454541
- Characterization of the biochemical properties of the human Upf1 gene product that is involved in nonsense-mediated mRNA decay. | Bhattacharya, A., et al. 2000. RNA. 6: 1226-35. PMID: 10999600
- Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes. | Mendell, JT., et al. 2000. Mol Cell Biol. 20: 8944-57. PMID: 11073994
- Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4). | Serin, G., et al. 2001. Mol Cell Biol. 21: 209-23. PMID: 11113196
- Rent1, a trans-effector of nonsense-mediated mRNA decay, is essential for mammalian embryonic viability. | Medghalchi, SM., et al. 2001. Hum Mol Genet. 10: 99-105. PMID: 11152657
- Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. | Kim, VN., et al. 2001. Science. 293: 1832-6. PMID: 11546873
- Mammalian orthologues of a yeast regulator of nonsense transcript stability. | Perlick, HA., et al. 1996. Proc Natl Acad Sci U S A. 93: 10928-32. PMID: 8855285
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rent3b Antibody (D-1) | sc-514564 | 200 µg/ml | $316.00 | |||
Rent3b Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-538662 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rent3b Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-536316 | 200 µg Ab; 40 µg BP | $354.00 | |||
Rent3b Antibody (D-1): m-IgG1 BP-HRP Bundle | sc-545818 | 200 µg Ab; 20 µg BP | $354.00 | |||
Rent3b (D-1) Neutralizing Peptide | sc-514564 P | 100 µg/0.5 ml | $68.00 |