Rent3 Antibody (E-8): sc-398821
- Rent3 Antibody (E-8) is a mouse monoclonal IgG1 κ Rent3 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 51-240 mapping near the N-terminus of Rent3b of human origin
- Rent3 Antibody (E-8) is recommended for detection of Rent3a and Rent3b of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- Anti-Rent3 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398821 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rent3 Antibody (E-8) for WB applications. These reagents are now offered in bundles with Rent3 Antibody (E-8) (see ordering information below).
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Rent3 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rent3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rent3 antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rent3 plays a crucial role in nonsense-mediated RNA decay (NMD), which maintains cellular integrity by preventing the translation of defective mRNA transcripts that could lead to truncated or malfunctioning proteins. This mechanism is particularly important in eukaryotic cells, where detecting and degrading such transcripts helps avoid potential deleterious effects on cellular function and organismal health. Rent3, along with mammalian orthologs Rent1 and Rent2, regulates RNA stability and processing, with Rent3 specifically interacting with spliced mRNA and proteins like Y14, linking splicing events to NMD pathways in the cytoplasm. Rent3′s primary localization to the nucleus, in contrast to Rent1 and Rent2′s predominantly cytoplasmic presence, highlights a specialized role in RNA metabolism and underscores important interactions in gene expression regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rent3 Antibody (E-8) References:
- SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast. | Page, MF., et al. 1999. Mol Cell Biol. 19: 5943-51. PMID: 10454541
- Characterization of the biochemical properties of the human Upf1 gene product that is involved in nonsense-mediated mRNA decay. | Bhattacharya, A., et al. 2000. RNA. 6: 1226-35. PMID: 10999600
- Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes. | Mendell, JT., et al. 2000. Mol Cell Biol. 20: 8944-57. PMID: 11073994
- Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4). | Serin, G., et al. 2001. Mol Cell Biol. 21: 209-23. PMID: 11113196
- Rent1, a trans-effector of nonsense-mediated mRNA decay, is essential for mammalian embryonic viability. | Medghalchi, SM., et al. 2001. Hum Mol Genet. 10: 99-105. PMID: 11152657
- Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. | Kim, VN., et al. 2001. Science. 293: 1832-6. PMID: 11546873
- Mammalian orthologues of a yeast regulator of nonsense transcript stability. | Perlick, HA., et al. 1996. Proc Natl Acad Sci U S A. 93: 10928-32. PMID: 8855285
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rent3 Antibody (E-8) | sc-398821 | 200 µg/ml | $322.00 | |||
Rent3 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-527817 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rent3 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-533190 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rent3 Antibody (E-8) X | sc-398821 X | 200 µg/0.1 ml | $322.00 | |||
Rent3 Antibody (E-8) AC | sc-398821 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rent3 Antibody (E-8) HRP | sc-398821 HRP | 200 µg/ml | $322.00 | |||
Rent3 Antibody (E-8) FITC | sc-398821 FITC | 200 µg/ml | $336.00 | |||
Rent3 Antibody (E-8) PE | sc-398821 PE | 200 µg/ml | $349.00 | |||
Rent3 Antibody (E-8) Alexa Fluor® 488 | sc-398821 AF488 | 200 µg/ml | $364.00 | |||
Rent3 Antibody (E-8) Alexa Fluor® 546 | sc-398821 AF546 | 200 µg/ml | $364.00 | |||
Rent3 Antibody (E-8) Alexa Fluor® 594 | sc-398821 AF594 | 200 µg/ml | $364.00 | |||
Rent3 Antibody (E-8) Alexa Fluor® 647 | sc-398821 AF647 | 200 µg/ml | $364.00 | |||
Rent3 Antibody (E-8) Alexa Fluor® 680 | sc-398821 AF680 | 200 µg/ml | $364.00 | |||
Rent3 Antibody (E-8) Alexa Fluor® 790 | sc-398821 AF790 | 200 µg/ml | $364.00 |