REEP5 Antibody (F-12)recommended for detection of REEP5 of human origin by WB, IP, IF, IHC(P) and ELISA

REEP5 Antibody (F-12): sc-393522

REEP5 Antibody (F-12) is rated 5.0 out of 5 by 1.
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Datasheets

    • REEP5 Antibody (F-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 114-189 mapping at the C-terminus of REEP5 of human origin
    • recommended for detection of REEP5 of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can REEP5 (F-12): sc-393522 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, REEP5 (F-12): sc-393522 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced positive Western blot data of REEP5 Produced positive Western blot data of REEP5 expression in non-transfected 293T, human REEP5 transfected 293T HeLa, HISM and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-07-21
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