RDH13 Antibody (E-2): sc-515447
- RDH13 Antibody (E-2) is a mouse monoclonal IgG1 κ RDH13 antibody provided at 200 µg/ml
- raised against amino acids 226-282 mapping within an internal region of RDH13 of human origin
- RDH13 Antibody (E-2) is recommended for detection of RDH13 of human origin by WB, IP, IF and ELISA
- Anti-RDH13 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RDH13 Antibody (E-2) for WB applications. These reagents are now offered in bundles with RDH13 Antibody (E-2) (see ordering information below).
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RDH13 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects RDH13 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RDH13 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RDH13, also known as all-trans and 9-cis retinol dehydrogenase 13 or SDR7C3, is a 331 amino acid mitochondrial protein that belongs to the short-chain dehydrogenases/reductases (SDR) family. RDH13 is widely expressed, particularly in the eye, pancreas, placenta, and lung, and is localized on the outer side of the inner mitochondrial membrane. RDH13 plays a crucial role in the metabolism of retinoids, recognizing them as substrates and potentially contributing to the production of retinoic acid, which is vital for various biological processes, including vision and cellular differentiation. RDH13 protects mitochondria from oxidative stress, highlighting its importance in maintaining cellular health. Defects in RDH13 have been linked to Leber congenital amaurosis (LCA) type 3, an inherited autosomal recessive retinal disease that represents the most common genetic cause of congenital visual impairment in infants and children, underscoring the significance of RDH13 in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RDH13 Antibody (E-2) References:
- Dual-substrate specificity short chain retinol dehydrogenases from the vertebrate retina. | Haeseleer, F., et al. 2002. J Biol Chem. 277: 45537-45546. PMID: 12226107
- Functional characterization of mouse RDH11 as a retinol dehydrogenase involved in dark adaptation in vivo. | Kasus-Jacobi, A., et al. 2005. J Biol Chem. 280: 20413-20. PMID: 15790565
- Microarray-based mutation detection and phenotypic characterization of patients with Leber congenital amaurosis. | Yzer, S., et al. 2006. Invest Ophthalmol Vis Sci. 47: 1167-76. PMID: 16505055
- RDH12, a retinol dehydrogenase causing Leber's congenital amaurosis, is also involved in steroid metabolism. | Keller, B. and Adamski, J. 2007. J Steroid Biochem Mol Biol. 104: 190-4. PMID: 17512723
- Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase/reductase with a retinaldehyde reductase activity. | Belyaeva, OV., et al. 2008. FEBS J. 275: 138-47. PMID: 18039331
- Highly efficient retinal metabolism in cones. | Miyazono, S., et al. 2008. Proc Natl Acad Sci U S A. 105: 16051-6. PMID: 18836074
- Genes on bovine chromosome 18 associated with bilateral convergent strabismus with exophthalmos in German Brown cattle. | Fink, S., et al. 2008. Mol Vis. 14: 1737-51. PMID: 18836565
- Medium- and short-chain dehydrogenase/reductase gene and protein families : Medium-chain and short-chain dehydrogenases/reductases in retinoid metabolism. | Parés, X., et al. 2008. Cell Mol Life Sci. 65: 3936-49. PMID: 19011747
- Proteome profile of functional mitochondria from human skeletal muscle using one-dimensional gel electrophoresis and HPLC-ESI-MS/MS. | Lefort, N., et al. 2009. J Proteomics. 72: 1046-60. PMID: 19567276
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RDH13 Antibody (E-2) | sc-515447 | 200 µg/ml | $322.00 | |||
RDH13 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-531372 | 200 µg Ab; 10 µg BP | $361.00 | |||
RDH13 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-525046 | 200 µg Ab, 40 µg BP | $361.00 | |||
RDH13 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-544511 | 200 µg Ab; 20 µg BP | $361.00 | |||
RDH13 Antibody (E-2) AC | sc-515447 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RDH13 Antibody (E-2) HRP | sc-515447 HRP | 200 µg/ml | $322.00 | |||
RDH13 Antibody (E-2) FITC | sc-515447 FITC | 200 µg/ml | $336.00 | |||
RDH13 Antibody (E-2) PE | sc-515447 PE | 200 µg/ml | $349.00 | |||
RDH13 Antibody (E-2) Alexa Fluor® 488 | sc-515447 AF488 | 200 µg/ml | $364.00 | |||
RDH13 Antibody (E-2) Alexa Fluor® 546 | sc-515447 AF546 | 200 µg/ml | $364.00 | |||
RDH13 Antibody (E-2) Alexa Fluor® 594 | sc-515447 AF594 | 200 µg/ml | $364.00 | |||
RDH13 Antibody (E-2) Alexa Fluor® 647 | sc-515447 AF647 | 200 µg/ml | $364.00 | |||
RDH13 Antibody (E-2) Alexa Fluor® 680 | sc-515447 AF680 | 200 µg/ml | $364.00 | |||
RDH13 Antibody (E-2) Alexa Fluor® 790 | sc-515447 AF790 | 200 µg/ml | $364.00 |