RCK Antibody (G-12): sc-166489

RCK Antibody (G-12) is a mouse monoclonal IgG2b antibody that detects RCK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RCK, also known as DDX6 or P54, is a crucial member of the DEAD-box RNA helicase family, characterized by common protein motifs and widespread presence in various tissues. RCK plays a vital role in RNA metabolism, functioning as an unwindase that exhibits ATP-dependent RNA unwinding activity and facilitates RNA decay in the 5′-3′ direction. RCK′s ability to regulate mRNA expression helps maintain normal transcriptional levels within the cell, preventing aberrant gene expression that could lead to uncontrolled cell growth. Notably, mutations in the gene encoding RCK can result in overexpression, transforming RCK into an oncogene that positively influences the expression of genes associated with cell proliferation. Understanding RCK′s intricate functions helps researchers appreciate its significance in both normal cellular processes and malignancy development, making anti-RCK antibody (G-12) an essential tool for studying RNA metabolism and its implications in health and disease.

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RCK Antibody (G-12) References:

1. Structural insight of human DEAD-box protein rck/p54 into its substrate recognition with conformational changes.  |  Matsui, T., et al. 2006. Genes Cells. 11: 439-52. PMID: 16611246
2. Dynamic oligomeric conversions of the cytoplasmic RCK domains mediate MthK potassium channel activity.  |  Kuo, MM., et al. 2007. Proc Natl Acad Sci U S A. 104: 2151-6. PMID: 17287352
3. The contribution of RCK domains to human BK channel allosteric activation.  |  Savalli, N., et al. 2012. J Biol Chem. 287: 21741-50. PMID: 22556415
4. Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation.  |  Smith, FJ., et al. 2012. Structure. 20: 2038-47. PMID: 23085076
5. Structural Studies of Potassium Transport Protein KtrA Regulator of Conductance of K+ (RCK) C Domain in Complex with Cyclic Diadenosine Monophosphate (c-di-AMP).  |  Kim, H., et al. 2015. J Biol Chem. 290: 16393-402. PMID: 25957408
6. A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy.  |  Hu, G., et al. 2015. Nat Cell Biol. 17: 930-942. PMID: 26098573
7. Identification of the epidermal growth factor receptor as the receptor for Salmonella Rck-dependent invasion.  |  Wiedemann, A., et al. 2016. FASEB J. 30: 4180-4191. PMID: 27609774
8. Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations.  |  Kim, S., et al. 2019. Nat Commun. 10: 3703. PMID: 31420535
9. Energetics of ångström-scale conformational changes in an RCK domain of the MthK K+ channel.  |  Lewis, JH. and Lu, Z. 2019. Nat Struct Mol Biol. 26: 808-815. PMID: 31488910
10. The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues.  |  Akao, Y., et al. 1995. Cancer Res. 55: 3444-9. PMID: 7614484

The preferred RCK antibody is RCK Antibody (E-12): sc-376433.