RCC2 Antibody (G-5): sc-514340
- RCC2 Antibody (G-5) is a mouse monoclonal IgG2a κ RCC2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 110-134 within an internal region of RCC2 of human origin
- RCC2 Antibody (G-5) is recommended for detection of RCC2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RCC2 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RCC2 Antibody (G-5) for WB applications. These reagents are now offered in bundles with RCC2 Antibody (G-5) (see ordering information below).
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RCC2 Antibody (G-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects RCC2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RCC2 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RCC2, also known as regulator of chromosome condensation 2, KIAA1470, or TD60, is a crucial 522 amino acid protein that plays a significant role in cell division. RCC2 is primarily localized to the nucleus, centromeres, and the midzone of the mitotic spindle in a cell-cycle dependent manner, which is vital for ensuring accurate chromosome segregation during mitosis. RCC2 interacts with Rac 1, functioning as a guanine nucleotide exchange factor that is essential for completing mitosis and cytokinesis. Additionally, RCC2 undergoes DNA damage-dependent phosphorylation, likely mediated by ATM or ATR, which highlights RCC2′s importance in maintaining genomic stability. The gene encoding RCC2 is located on human chromosome 1, a region that spans 260 million base pairs and contains over 3,000 genes, many of which are associated with various diseases, including familial adenomatous polyposis, Stickler syndrome, and several cancers. The aberrations in chromosome 1 are linked to a range of malignancies, underscoring RCC2′s significance in both normal cellular function and disease pathology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RCC2 Antibody (G-5) References:
- The mammalian passenger protein TD-60 is an RCC1 family member with an essential role in prometaphase to metaphase progression. | Mollinari, C., et al. 2003. Dev Cell. 5: 295-307. PMID: 12919680
- Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. | Yang, F., et al. 2007. J Cell Sci. 120: 4060-70. PMID: 17971412
- Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation. | Rosasco-Nitcher, SE., et al. 2008. Science. 319: 469-72. PMID: 18218899
- Common variants on 1p36 and 1q42 are associated with cutaneous basal cell carcinoma but not with melanoma or pigmentation traits. | Stacey, SN., et al. 2008. Nat Genet. 40: 1313-8. PMID: 18849993
- Telophase disc: a new mammalian mitotic organelle that bisects telophase cells with a possible function in cytokinesis. | Andreassen, PR., et al. 1991. J Cell Sci. 99 (Pt 3): 523-34. PMID: 1939370
- Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis. | Martineau-Thuillier, S., et al. 1998. Chromosoma. 107: 461-70. PMID: 9914378
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RCC2 Antibody (G-5) | sc-514340 | 200 µg/ml | $322.00 | |||
RCC2 Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-526359 | 200 µg Ab; 10 µg BP | $361.00 | |||
RCC2 Antibody (G-5): m-IgG2a BP-HRP Bundle | sc-546603 | 200 µg Ab; 10 µg BP | $361.00 | |||
RCC2 Antibody (G-5) AC | sc-514340 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RCC2 Antibody (G-5) HRP | sc-514340 HRP | 200 µg/ml | $322.00 | |||
RCC2 Antibody (G-5) FITC | sc-514340 FITC | 200 µg/ml | $336.00 | |||
RCC2 Antibody (G-5) PE | sc-514340 PE | 200 µg/ml | $349.00 | |||
RCC2 Antibody (G-5) Alexa Fluor® 488 | sc-514340 AF488 | 200 µg/ml | $364.00 | |||
RCC2 Antibody (G-5) Alexa Fluor® 546 | sc-514340 AF546 | 200 µg/ml | $364.00 | |||
RCC2 Antibody (G-5) Alexa Fluor® 594 | sc-514340 AF594 | 200 µg/ml | $364.00 | |||
RCC2 Antibody (G-5) Alexa Fluor® 647 | sc-514340 AF647 | 200 µg/ml | $364.00 | |||
RCC2 Antibody (G-5) Alexa Fluor® 680 | sc-514340 AF680 | 200 µg/ml | $364.00 | |||
RCC2 Antibody (G-5) Alexa Fluor® 790 | sc-514340 AF790 | 200 µg/ml | $364.00 | |||
RCC2 (G-5) Neutralizing Peptide | sc-514340 P | 100 µg/0.5 ml | $69.00 |