RBP-Jκ Antibody (E-7): sc-271128

RBP-Jκ Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects RBP-Jκ protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RBP-Jκ (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Recombination Signal Binding Protein Jκ (RBP-Jκ), also known as KBF2 or CBF1, plays a crucial role in gene expression regulation and is essential for the Notch signaling pathway, which drives cell differentiation and development. RBP-Jκ predominantly resides in the nucleus, functioning as a transcriptional regulator by binding to DNA and interacting with the cleaved intracellular domain of the Notch 1 receptor. RBP-Jκ′s nuclear localization enables modulation of target genes, including NFκB p52 and the Epstein-Barr virus (EBV) protein EBNA-2, both critical for various cellular processes and immune responses. RBP-Jκ′s interaction with Notch 1 underscores RBP-Jκ′s significance in cellular communication and development, making anti-RBP-Jκ antibody (E-7) an invaluable tool for researchers studying gene regulation and signaling pathways in mammalian systems.

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RBP-Jκ Antibody (E-7) References:

1. Enhanced expression and autoimmunity of recombination signal binding protein-jkappa in human dilated cardiomyopathy.  |  Nickenig, G., et al. 1999. Biochem Biophys Res Commun. 266: 432-6. PMID: 10600520
2. The Ku antigen-recombination signal-binding protein Jkappa complex binds to the nuclear factor-kappaB p50 promoter and acts as a positive regulator of p50 expression in human gastric cancer cells.  |  Lim, JW., et al. 2004. J Biol Chem. 279: 231-7. PMID: 14570916
3. Disruption of the transcription factor recombination signal-binding protein-Jkappa (RBP-J) leads to veno-occlusive disease and interfered liver regeneration in mice.  |  Wang, L., et al. 2009. Hepatology. 49: 268-77. PMID: 19065680
4. Myeloid-specific disruption of recombination signal binding protein Jκ ameliorates hepatic fibrosis by attenuating inflammation through cylindromatosis in mice.  |  He, F., et al. 2015. Hepatology. 61: 303-14. PMID: 25145286
5. A herpesvirus transactivator and cellular POU proteins extensively regulate DNA binding of the host Notch signaling protein RBP-Jκ to the virus genome.  |  Gonzalez-Lopez, O., et al. 2019. J Biol Chem. 294: 13073-13092. PMID: 31308175
6. Disruption of the mouse RBP-J kappa gene results in early embryonic death.  |  Oka, C., et al. 1995. Development. 121: 3291-301. PMID: 7588063
7. The human J kappa recombination signal sequence binding protein (RBP-J kappa) targets the Epstein-Barr virus EBNA2 protein to its DNA responsive elements.  |  Waltzer, L., et al. 1994. EMBO J. 13: 5633-8. PMID: 7988560
8. Human Jk recombination signal binding protein gene (IGKJRB): comparison with its mouse homologue.  |  Amakawa, R., et al. 1993. Genomics. 17: 306-15. PMID: 8406481
9. RBP-J kappa repression activity is mediated by a co-repressor and antagonized by the Epstein-Barr virus transcription factor EBNA2.  |  Waltzer, L., et al. 1995. Nucleic Acids Res. 23: 4939-45. PMID: 8559649
10. Truncated mammalian Notch1 activates CBF1/RBPJk-repressed genes by a mechanism resembling that of Epstein-Barr virus EBNA2.  |  Hsieh, JJ., et al. 1996. Mol Cell Biol. 16: 952-9. PMID: 8622698
11. Physical interaction between a novel domain of the receptor Notch and the transcription factor RBP-J kappa/Su(H).  |  Tamura, K., et al. 1995. Curr Biol. 5: 1416-23. PMID: 8749394
12. NF-kappaB2 is a putative target gene of activated Notch-1 via RBP-Jkappa.  |  Oswald, F., et al. 1998. Mol Cell Biol. 18: 2077-88. PMID: 9528780

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RBP-Jκ Antibody (E-7) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-RBP-Jκ Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RBP-Jκ Antibody (E-7) Specifications

Alternate Product Name: RBPJ

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 254-273

Epitope Target: RBP-Jκ

Epitope Species: mouse

Product Reactivity/Detection: RBP-Jκ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for RBP-Jκ Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin