RBM17 Antibody (F-5): sc-515587
- RBM17 Antibody (F-5) is a mouse monoclonal IgG2a κ RBM17 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 93-117 within an internal region of RBM17 of human origin
- RBM17 Antibody (F-5) is recommended for detection of RBM17 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RBM17 Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RBM17 Antibody (F-5) for WB applications. These reagents are now offered in bundles with RBM17 Antibody (F-5) (see ordering information below).
RBM17 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects RBM17 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RBM17 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RBM17, also known as SPF45 (splicing factor 45), is a 401 amino acid protein that plays a crucial role in the nucleus, where it contains one G-patch domain and one RNA recognition motif (RRM) domain. RBM17 is integral to the spliceosome complex, functioning as a splicing factor that binds to specific regions at the intron/exon borders, thereby regulating alternative splicing and the utilization of cryptic splice sites. The importance of RBM17 in alternative splicing is underscored by its involvement in various cellular processes, including gene expression regulation and the generation of protein diversity, which are essential for normal cellular function and development. The gene encoding RBM17 is located on human chromosome 10, a region associated with several genetic disorders, including Charcot-Marie-Tooth disease and multiple endocrine neoplasia type 2, highlighting the clinical significance of RBM17 in human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RBM17 Antibody (F-5) References:
- Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45. | Lallena, MJ., et al. 2002. Cell. 109: 285-96. PMID: 12015979
- Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. | Will, CL., et al. 2002. EMBO J. 21: 4978-88. PMID: 12234937
- Human SPF45, a splicing factor, has limited expression in normal tissues, is overexpressed in many tumors, and can confer a multidrug-resistant phenotype to cells. | Sampath, J., et al. 2003. Am J Pathol. 163: 1781-90. PMID: 14578179
- Human splicing factor SPF45 (RBM17) confers broad multidrug resistance to anticancer drugs when overexpressed--a phenotype partially reversed by selective estrogen receptor modulators. | Perry, WL., et al. 2005. Cancer Res. 65: 6593-600. PMID: 16061639
- U2AF-homology motif interactions are required for alternative splicing regulation by SPF45. | Corsini, L., et al. 2007. Nat Struct Mol Biol. 14: 620-9. PMID: 17589525
- Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1. | Lim, J., et al. 2008. Nature. 452: 713-8. PMID: 18337722
- Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. | Neubauer, G., et al. 1998. Nat Genet. 20: 46-50. PMID: 9731529
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM17 Antibody (F-5) | sc-515587 | 200 µg/ml | $322.00 | |||
RBM17 Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-531429 | 200 µg Ab; 10 µg BP | $361.00 | |||
RBM17 Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-525117 | 200 µg Ab, 40 µg BP | $361.00 | |||
RBM17 Antibody (F-5): m-IgG2a BP-HRP Bundle | sc-547788 | 200 µg Ab; 10 µg BP | $361.00 | |||
RBM17 Antibody (F-5) AC | sc-515587 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RBM17 Antibody (F-5) HRP | sc-515587 HRP | 200 µg/ml | $322.00 | |||
RBM17 Antibody (F-5) FITC | sc-515587 FITC | 200 µg/ml | $336.00 | |||
RBM17 Antibody (F-5) PE | sc-515587 PE | 200 µg/ml | $349.00 | |||
RBM17 Antibody (F-5) Alexa Fluor® 488 | sc-515587 AF488 | 200 µg/ml | $364.00 | |||
RBM17 Antibody (F-5) Alexa Fluor® 546 | sc-515587 AF546 | 200 µg/ml | $364.00 | |||
RBM17 Antibody (F-5) Alexa Fluor® 594 | sc-515587 AF594 | 200 µg/ml | $364.00 | |||
RBM17 Antibody (F-5) Alexa Fluor® 647 | sc-515587 AF647 | 200 µg/ml | $364.00 | |||
RBM17 Antibody (F-5) Alexa Fluor® 680 | sc-515587 AF680 | 200 µg/ml | $364.00 | |||
RBM17 Antibody (F-5) Alexa Fluor® 790 | sc-515587 AF790 | 200 µg/ml | $364.00 | |||
RBM17 (F-5) Neutralizing Peptide | sc-515587 P | 100 µg/0.5 ml | $69.00 |