Rb Antibody (Rb1): sc-73598

Rb Antibody (Rb1) is a mouse monoclonal IgG1 kappa light chain antibody raised against full-length human retinoblastoma β-galactosidase fusion protein, a critical tumor suppressor involved in cell cycle regulation. Rb mouse monoclonal antibody (Rb1) recognizes an epitope within the Rb p110 immunogen sequence and demonstrates broad species cross-reactivity, detecting Rb in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunohistochemistry on paraffin-embedded sections (IHCP), and flow cytometry (FCM) applications. Anti-Rb monoclonal antibody (Rb1) is available in both conjugated and non-conjugated forms. The retinoblastoma protein functions as a sophisticated regulator of cell cycle progression, particularly at the G1/S transition, through complex interactions with E2F transcription factors that control differentiation, apoptosis, and cell proliferation. In hypophosphorylated form, Rb forms repressive complexes with E2F factors, actively silencing cell cycle-related genes through two main mechanisms: recruitment of histone deacetylase and SWI/SNF chromatin remodeling complexes that promote chromatin condensation. This repression undergoes precise regulation through phosphorylation by cyclin D-dependent kinases (D1, D2, D3) at multiple sites, including Ser249/Thr252, Thr373, Thr356, Ser780, Ser795, Ser807/Ser811, and Thr821/Thr826. These modifications convert Rb to an inactive state, releasing E2F factors to activate S phase genes. Beyond direct transcriptional control, Rb associates with histone methyltransferases and Polycomb group proteins, expanding Rb′s influence over chromatin structure and gene expression. Mutations in the RB1 gene encoding Rb can lead to pediatric retinoblastoma and contribute to various human tumors by disrupting these regulatory mechanisms. Through these diverse cellular functions, Rb (Rb1) antibody serves as a valuable tool for investigating cell cycle control, tumor suppression pathways, and molecular mechanisms of cancer development.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rb Antibody (Rb1) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Rb Antibody (Rb1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rb Antibody (Rb1) Specifications

Alternate Product Name: RB1

Product Clone Name: Rb1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 703-722

Epitope Target: Rb

Epitope Species: human

Product Reactivity/Detection: Rb p110

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Rb Antibody (Rb1): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred Rb antibody is Rb Antibody (IF8): sc-102.