Rb Antibody (C-2): sc-74562
- Rb Antibody (C-2) is a mouse monoclonal IgG2a κ, cited in 38 publications, provided at 200 µg/ml
- raised against amino acids 769-921 mapping at the C-terminus of Rb of mouse origin
- Anti-Rb Antibody (C-2) is recommended for detection of Rb of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Rb Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Rb Antibody (C-2) for WB applications. This reagent is now offered in a bundle with Rb Antibody (C-2) (see ordering information below).
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SEE ALSO...
Rb Antibody (C-2) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 769-921 at the C-terminus of mouse retinoblastoma protein (Rb), a crucial cell cycle regulator and tumor suppressor. Rb mouse monoclonal antibody (C-2) recognizes an epitope within the specified immunogen sequence and demonstrates broad species cross-reactivity, detecting Rb in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-Rb monoclonal antibody (C-2) is available in non-conjugated form and multiple conjugated forms including agarose, HRP, PE, FITC, and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The retinoblastoma protein, encoded by the RB1 gene on chromosome 13q14.2, undergoes complex regulation through phosphorylation to control cell cycle progression, differentiation, and apoptosis. In hypophosphorylated form, Rb binds to E2F transcription factors, actively repressing genes required for S-phase entry. Multiple phosphorylation events by cyclin D-dependent kinases at specific residues (Ser249/Thr252, Thr373, Thr356, Ser780, Ser795, Ser807/Ser811, and Thr821/Thr826) trigger Rb inactivation and E2F release, promoting cell cycle advancement. Beyond direct transcriptional control, Rb recruits histone deacetylase complexes to target promoters, inducing chromatin condensation and enhancing transcriptional repression. The RB1 gene produces multiple splice variants, generating various Rb isoforms with potentially distinct functions based on their domain composition and ability to interact with regulatory partners. Mutations or dysregulation of RB1 can compromise Rb′s tumor suppressor functions, contributing to retinoblastoma and other human cancers. Through these diverse cellular roles, Rb (C-2) antibody serves as a valuable tool for investigating cell cycle regulation, differentiation, apoptosis, and tumorigenesis mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rb Antibody (C-2) | sc-74562 | 200 µg/ml | $316.00 | |||
Rb Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-525730 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rb Antibody (C-2) AC | sc-74562 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Rb Antibody (C-2) HRP | sc-74562 HRP | 200 µg/ml | $316.00 | |||
Rb Antibody (C-2) FITC | sc-74562 FITC | 200 µg/ml | $330.00 | |||
Rb Antibody (C-2) PE | sc-74562 PE | 200 µg/ml | $343.00 | |||
Rb Antibody (C-2) Alexa Fluor® 488 | sc-74562 AF488 | 200 µg/ml | $357.00 | |||
Rb Antibody (C-2) Alexa Fluor® 546 | sc-74562 AF546 | 200 µg/ml | $357.00 | |||
Rb Antibody (C-2) Alexa Fluor® 594 | sc-74562 AF594 | 200 µg/ml | $357.00 | |||
Rb Antibody (C-2) Alexa Fluor® 647 | sc-74562 AF647 | 200 µg/ml | $357.00 | |||
Rb Antibody (C-2) Alexa Fluor® 680 | sc-74562 AF680 | 200 µg/ml | $357.00 | |||
Rb Antibody (C-2) Alexa Fluor® 790 | sc-74562 AF790 | 200 µg/ml | $357.00 |