Ras-GRF1 Antibody (D-12): sc-377234

Ras-GRF1 Antibody (D-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ras-GRF1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Ras-GRF1 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ras-GRF1 plays a pivotal role in regulating Ras signaling pathways, which are crucial for cell growth, differentiation, and survival. Specifically, Ras-GRF1 functions as a guanine nucleotide exchange factor (GEF) that facilitates converting Ras proteins from their inactive GDP-bound state to their active GTP-bound form, a key step in signal transduction initiated by cell surface receptor activation. This activation is essential for downstream signaling cascades that influence various cellular processes, including proliferation and apoptosis. Ras-GRF1 exhibits specificity for H- and N-Ras proteins, enhancing GDP release, while showing no effect on related Ral A or Cdc42Hs proteins. Structural similarities between Ras-GRF1 and other GEFs, such as human breakpoint cluster protein and Dbl proto-oncogene product, underscore Ras-GRF1′s importance in cellular signaling networks. Understanding Ras-GRF1 function and interactions is vital for elucidating mechanisms underlying various diseases, including cancer, where Ras signaling is often dysregulated.

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Ras-GRF1 Antibody (D-12) References:

1. Regulation of DNA methylation of Rasgrf1.  |  Yoon, BJ., et al. 2002. Nat Genet. 30: 92-6. PMID: 11753386
2. Ras-GRF1 signaling is required for normal beta-cell development and glucose homeostasis.  |  Font de Mora, J., et al. 2003. EMBO J. 22: 3039-49. PMID: 12805218
3. Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.  |  Arozarena, I., et al. 2004. Mol Cell Biol. 24: 1516-30. PMID: 14749369
4. Rasgrf1 imprinting is regulated by a CTCF-dependent methylation-sensitive enhancer blocker.  |  Yoon, B., et al. 2005. Mol Cell Biol. 25: 11184-90. PMID: 16314537
5. Distinct roles for Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and Ras-GRF2 in the induction of long-term potentiation and long-term depression.  |  Li, S., et al. 2006. J Neurosci. 26: 1721-9. PMID: 16467520
6. The Ras-GRF1 exchange factor coordinates activation of H-Ras and Rac1 to control neuronal morphology.  |  Yang, H. and Mattingly, RR. 2006. Mol Biol Cell. 17: 2177-89. PMID: 16481401
7. The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction.  |  Forlani, G., et al. 2006. FEBS J. 273: 2127-38. PMID: 16649990
8. Filamin A-mediated down-regulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cells.  |  Zhu, TN., et al. 2007. J Biol Chem. 282: 14816-26. PMID: 17389601
9. Ras-pathway has a dual role in yeast galactose metabolism.  |  Mirisola, MG., et al. 2007. FEBS Lett. 581: 2009-16. PMID: 17475260
10. Antagonism between DNA and H3K27 methylation at the imprinted Rasgrf1 locus.  |  Lindroth, AM., et al. 2008. PLoS Genet. 4: e1000145. PMID: 18670629
11. Differential involvement of Ras-GRF1 and Ras-GRF2 in L-DOPA-induced dyskinesia.  |  Bido, S., et al. 2015. Ann Clin Transl Neurol. 2: 662-78. PMID: 26125041
12. The Rasgrf1-repeat sequence (D9Ncvs53) maps between Mod1 and Rbp1 on mouse chromosome 9 and may define a putative imprinted region.  |  Pearsall, RS., et al. 1998. Mamm Genome. 9: 261-2. PMID: 9501318

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ras-GRF1 Antibody (D-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Ras-GRF1 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ras-GRF1 Antibody (D-12) Specifications

Alternate Product Name: Ras-GRF1

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 791-1262

Epitope Target: Ras-GRF1 p140 and Ras-GRF2

Epitope Species: mouse

Product Reactivity/Detection: Ras-GRF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Ras-GRF1 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin