Date published: 2025-9-5

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Raptor 抗体 (A-2): sc-518004

4.5(2)
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说明书
  • Raptor抗体(A-2)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的的N-terminus附近的氨基酸84-109之间
  • 抗-Raptor 抗体 (A-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Raptor isoforms 1, 2 and 3
  • 抗Raptor抗体(A-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Raptor (A-2): sc-518004免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Raptor Antibody (A-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Raptor Antibody (A-2) 打包提供(请参阅下面的订购信息)。

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関連項目

Raptor抗体(A-2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的Raptor。Raptor (A-2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。FRAP的调节相关蛋白被称为Raptor,在哺乳动物雷帕霉素靶蛋白(mTOR)信号通路中起着关键作用,而mTOR信号通路对于细胞生长和代谢至关重要。Raptor主要位于细胞质中,与mTOR相互作用形成对营养物质供应敏感的复合物。这种定位非常重要,因为Raptor充当细胞营养状况的传感器,从而调节mTOR活性,影响蛋白质合成和细胞生长等过程,以应对环境变化。在营养丰富的条件下,Raptor促进mTOR活性,促进下游靶标(如4E-BP1)的磷酸化,这对依赖帽的翻译至关重要。相反,在营养缺乏的情况下,Raptor作为mTOR的负调控因子,有助于节约资源并保持细胞内环境稳定。Raptor主要在骨骼肌中表达,在脑、肾、肺和胎盘中的表达水平较低,这表明Raptor在代谢需求高的组织中非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Raptor 抗体 (A-2)

sc-518004
200 µg/ml
$316.00

Raptor (A-2): m-IgG Fc BP-HRP 套装

sc-531587
200 µg Ab; 10 µg BP
$354.00

Raptor (A-2): m-IgGκ BP-HRP 套装

sc-525318
200 µg Ab, 40 µg BP
$354.00

Raptor (A-2): m-IgG1 BP-HRP 套装

sc-544643
200 µg Ab; 20 µg BP
$354.00

Raptor 抗体 (A-2) AC

sc-518004 AC
500 µg/ml, 25% agarose
$416.00

Raptor 抗体 (A-2) HRP

sc-518004 HRP
200 µg/ml
$316.00

Raptor 抗体 (A-2) FITC

sc-518004 FITC
200 µg/ml
$330.00

Raptor 抗体 (A-2) PE

sc-518004 PE
200 µg/ml
$343.00

Raptor 抗体 (A-2) Alexa Fluor® 488

sc-518004 AF488
200 µg/ml
$357.00

Raptor 抗体 (A-2) Alexa Fluor® 546

sc-518004 AF546
200 µg/ml
$357.00

Raptor 抗体 (A-2) Alexa Fluor® 594

sc-518004 AF594
200 µg/ml
$357.00

Raptor 抗体 (A-2) Alexa Fluor® 647

sc-518004 AF647
200 µg/ml
$357.00

Raptor 抗体 (A-2) Alexa Fluor® 680

sc-518004 AF680
200 µg/ml
$357.00

Raptor 抗体 (A-2) Alexa Fluor® 790

sc-518004 AF790
200 µg/ml
$357.00

Does this antibody or any Santa Cruz antibody for Raptor work very well in Mouse tissues?

Asked by: IT416
Thank you for your question. sc-518004 Raptor (A-2) is recommended for mouse, rat and human samples.
Answered by: BlakeJ
Date published: 2024-12-19

Has anybody used this antibody successfully to detect endogenous levels of Raptor by IFA and WB in human cell lines (eg. HeLa)?

Asked by: cmakh
Thank you for your question. We show data from endogenous expression of Raptor in human samples on the product page: Human glioblastoma astrocytoma cell line U-251 MG and human pancreas cell line PANC-1. We do not have image data for IF use, but the antibody has been established in this application using our standard protocols and is recommended for it.
Answered by: Tech Support Europe
Date published: 2020-08-28
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Rated 5 out of 5 by from Antibody works well by IHC in human tissueAntibody detects Raptor by Immunohistochemistry, in formalin-fixed, paraffin-embedded human esophagus tissue, yielding cytoplasmic staining of squamous epithelial cells. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from need further confirmI didn't get band at 100-200kDa in WB. I will try again.
Date published: 2017-12-29
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Raptor 抗体 (A-2) is rated 4.5 out of 5 by 2.
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