Raptor Antibody (10E10): sc-81537

Raptor Antibody (10E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Raptor protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Raptor antibody (10E10) is available as a non-conjugated format. Raptor protein, also known as regulatory associated protein of FRAP, plays a pivotal role in the mammalian target of rapamycin (mTOR) signaling pathway, which is crucial for regulating cell growth, proliferation, and survival in response to nutrient availability. Specifically, Raptor acts as a scaffold that facilitates interaction between mTOR and substrates, including 4E-BP1, thereby promoting phosphorylation and activation. This interaction is vital for cellular response to nutrient levels, as Raptor-mTOR complex is sensitive to changes in nutrient status, allowing cells to adapt growth and metabolism accordingly. Under nutrient-rich conditions, Raptor enhances mTOR activity, while in nutrient-deprived states, Raptor serves as a negative regulator, inhibiting mTOR signaling to conserve resources. Raptor is predominantly expressed in skeletal muscle, with lower levels found in brain, kidney, lung, and placenta, highlighting importance in tissues with high metabolic demands.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Raptor Antibody (10E10) References:

1. mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery.  |  Kim, DH., et al. 2002. Cell. 110: 163-75. PMID: 12150925
2. Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.  |  Hara, K., et al. 2002. Cell. 110: 177-89. PMID: 12150926
3. The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.  |  Nojima, H., et al. 2003. J Biol Chem. 278: 15461-4. PMID: 12604610
4. Raptor and mTOR: subunits of a nutrient-sensitive complex.  |  Kim, DH. and Sabatini, DM. 2004. Curr Top Microbiol Immunol. 279: 259-70. PMID: 14560962
5. Raptor, a binding partner of target of rapamycin.  |  Yonezawa, K., et al. 2004. Biochem Biophys Res Commun. 313: 437-41. PMID: 14684181
6. Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function.  |  Oshiro, N., et al. 2004. Genes Cells. 9: 359-66. PMID: 15066126
7. Structure of S6 kinase 1 determines whether raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site.  |  Ali, SM. and Sabatini, DM. 2005. J Biol Chem. 280: 19445-8. PMID: 15809305
8. Redox regulation of the nutrient-sensitive raptor-mTOR pathway and complex.  |  Sarbassov, DD. and Sabatini, DM. 2005. J Biol Chem. 280: 39505-9. PMID: 16183647
9. AMPK phosphorylation of raptor mediates a metabolic checkpoint.  |  Gwinn, DM., et al. 2008. Mol Cell. 30: 214-26. PMID: 18439900
10. The role of Raptor in lymphocytes differentiation and function.  |  Tang, J., et al. 2023. Front Immunol. 14: 1146628. PMID: 37283744
11. Cathepsin K inhibition induces Raptor destabilization and mitochondrial dysfunction via Syk/SHP2/Src/OTUB1 axis-mediated signaling.  |  Seo, SU., et al. 2023. Cell Death Dis. 14: 366. PMID: 37330581
12. O-GlcNAcylation of Raptor transduces glucose signals to mTORC1.  |  Xu, C., et al. 2023. Mol Cell. 83: 3027-3040.e11. PMID: 37541260

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Raptor Antibody (10E10) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-Raptor Antibody (10E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Raptor Antibody (10E10) Specifications

Alternate Product Name: Raptor

Product Clone Name: 10E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Raptor

Epitope Species: human

Product Reactivity/Detection: Raptor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for Raptor Antibody (10E10): N/A