Ran Antibody (A-7): sc-271376

Ran Antibody (A-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ran protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Ran antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ran, a small Ras-related GTPase, plays a crucial role in regulating nucleocytoplasmic transport, which maintains cellular homeostasis and facilitates proper functioning of cellular processes like DNA replication and mitosis. Located predominantly in the nucleus, Ran is involved in transporting RNA and proteins through the nuclear pore complex, ensuring essential molecules are accurately shuttled between the nucleus and cytoplasm. Ran activity is tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs), with RCC1 serving as a key GEF that promotes GDP to GTP exchange, thereby activating Ran. Ran GAP1 enhances GTP to GDP hydrolysis, maintaining the balance of active and inactive forms. Additionally, proteins such as Ran BP-1 and Ran BP-2 interact with Ran, influencing activity and localization, which underscores Ran′s importance in cellular dynamics and signaling pathways.

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Ran Antibody (A-7) References:

1. A monoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells.  |  Hieda, M., et al. 1999. J Cell Biol. 144: 645-55. PMID: 10037787
2. Directed inhibition of nuclear import in cellular hypertrophy.  |  Perez-Terzic, C., et al. 2001. J Biol Chem. 276: 20566-71. PMID: 11283025
3. Inter-cellular transport of ran GTPase.  |  Khuperkar, D., et al. 2015. PLoS One. 10: e0125506. PMID: 25894517
4. Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.  |  Nguyen, L., et al. 2020. Neuron. 105: 645-662.e11. PMID: 31831332
5. RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases.  |  Audia, S., et al. 2023. Cancers (Basel). 15: PMID: 36672435
6. GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.  |  Melchior, F., et al. 1995. J Cell Biol. 131: 571-81. PMID: 7593180
7. The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif.  |  Beddow, AL., et al. 1995. Proc Natl Acad Sci U S A. 92: 3328-32. PMID: 7724562
8. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
9. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1.  |  Klebe, C., et al. 1995. Biochemistry. 34: 639-47. PMID: 7819259
10. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.  |  Bischoff, FR., et al. 1995. EMBO J. 14: 705-15. PMID: 7882974
11. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.  |  Scheffzek, K., et al. 1995. Nature. 374: 378-81. PMID: 7885480
12. Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.  |  Ren, M., et al. 1995. Mol Cell Biol. 15: 2117-24. PMID: 7891706

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ran Antibody (A-7) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-Ran Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ran Antibody (A-7) Specifications

Alternate Product Name: Ran

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 121-216

Epitope Target: Ran

Epitope Species: human

Product Reactivity/Detection: Ran

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for Ran Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin