RAMP2 Antibody (B-5): sc-365240

RAMP2 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects RAMP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RAMP2 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RAMP2 plays a crucial role in modulating receptor activity, particularly in interactions with calcitonin receptor-like receptors (CRLR). By associating with CRLR in the endoplasmic reticulum, RAMP2 facilitates proper glycosylation and transport of CRLR to the cell surface, where CRLR functions as a receptor for vasodilatory peptides such as adrenomedullin (ADM). This interaction is vital for cardiovascular regulation and maintenance of vascular homeostasis, as ADM influences blood pressure and vascular tone. RAMP2, along with RAMP-1 and RAMP-3, exhibits structural similarities as type I receptors, characterized by a single extracellular N-terminus and cytoplasmic C-terminus, sharing approximately 55% sequence similarity. While RAMP-1 is predominantly expressed in uterus, brain, and gastrointestinal tract, RAMP-2 and RAMP-3 show higher expression levels in lung, breast, and fetal tissues, highlighting diverse physiological roles across different organ systems.

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RAMP2 Antibody (B-5) References:

1. The RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells.  |  Kamitani, S., et al. 1999. FEBS Lett. 448: 111-4. PMID: 10217420
2. A receptor activity modifying protein (RAMP)2-dependent adrenomedullin receptor is a calcitonin gene-related peptide receptor when coexpressed with human RAMP1.  |  Bühlmann, N., et al. 1999. Endocrinology. 140: 2883-90. PMID: 10342881
3. The amino terminus of receptor activity modifying proteins is a critical determinant of glycosylation state and ligand binding of calcitonin receptor-like receptor.  |  Fraser, NJ., et al. 1999. Mol Pharmacol. 55: 1054-9. PMID: 10347248
4. RAMPs: accessory proteins for seven transmembrane domain receptors.  |  Foord, SM. and Marshall, FH. 1999. Trends Pharmacol Sci. 20: 184-7. PMID: 10354609
5. Adrenomedullin-RAMP2 System in Vascular Endothelial Cells.  |  Koyama, T., et al. 2015. J Atheroscler Thromb. 22: 647-53. PMID: 26040754
6. Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress.  |  Cui, N., et al. 2021. Endocrinology. 162: PMID: 33545715
7. RAMP2-AS1 Regulates Endothelial Homeostasis and Aging.  |  Lai, CH., et al. 2021. Front Cell Dev Biol. 9: 635307. PMID: 33644072
8. Negative allosteric modulation of the glucagon receptor by RAMP2.  |  Krishna Kumar, K., et al. 2023. Cell. 186: 1465-1477.e18. PMID: 37001505
9. Balance of Gata3 and Ramp2 in hepatocytes regulates hepatic vascular reconstitution in postoperative liver regeneration.  |  Wang, B., et al. 2024. J Hepatol. 80: 309-321. PMID: 37918568
10. RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor.  |  McLatchie, LM., et al. 1998. Nature. 393: 333-9. PMID: 9620797
11. Expression of calcitonin receptor-like receptor and receptor-activity-modifying proteins in human cranial arteries.  |  Sams, A. and Jansen-Olesen, I. 1998. Neurosci Lett. 258: 41-4. PMID: 9876047
12. Desensitization of CGRP and adrenomedullin receptors in SK-N-MC cells: implications for the RAMP hypothesis.  |  Drake, WM., et al. 1999. Endocrinology. 140: 533-7. PMID: 9886866

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RAMP2 Antibody (B-5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-RAMP2 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RAMP2 Antibody (B-5) Specifications

Alternate Product Name: RAMP2

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 28-166

Epitope Target: RAMP2

Epitope Species: human

Product Reactivity/Detection: RAMP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for RAMP2 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin