Rad9 Antibody (B-6): sc-166457
- Rad9 Antibody (B-6) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 371-390 at the C-terminus of Rad9 of human origin
- recommended for detection of Rad9 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rad9 Antibody (B-6) for WB applications. These reagents are now offered in bundles with Rad9 Antibody (B-6) (see ordering information below).
Rad9 Antibody (B-6) is a mouse monoclonal IgG1 antibody that detects Rad9 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad9 plays a crucial role in the DNA damage response, particularly at the G2 checkpoint, where Rad9 is involved in the signal transduction cascade that regulates cell cycle arrest. Rad9 is essential for maintaining genomic stability, helping to prevent cell cycle progression when DNA damage is present, allowing repair mechanisms to take place. Rad9 undergoes extensive phosphorylation in vivo, enhancing interactions with other checkpoint control proteins, such as Rad1 and Hus1, forming a complex vital for effective DNA damage signaling. Additionally, Rad9′s association with anti-apoptotic Bcl-2 family proteins, including Bcl-2 and Bcl-xL, suggests a role in regulating apoptosis following DNA damage, highlighting Rad9′s importance in both cell cycle regulation and cellular stress responses. anti-Rad9 antibody (B-6) serves as an invaluable tool for researchers studying DNA damage response and cell cycle regulation mechanisms.
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Rad9 Antibody (B-6) References:
- The cell cycle checkpoint gene Rad9 is a novel oncogene activated by 11q13 amplification and DNA methylation in breast cancer. | Cheng, CK., et al. 2005. Cancer Res. 65: 8646-54. PMID: 16204032
- The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1. | Delacroix, S., et al. 2007. Genes Dev. 21: 1472-7. PMID: 17575048
- Rad9 has a functional role in human prostate carcinogenesis. | Zhu, A., et al. 2008. Cancer Res. 68: 1267-74. PMID: 18316588
- Rad9 is upregulated and plays protective roles in an acute lung injury model. | Yamamoto, M., et al. 2008. Biochem Biophys Res Commun. 376: 590-4. PMID: 18809378
- Cell cycle-dependent processing of DNA lesions controls localization of Rad9 to sites of genotoxic stress. | Warmerdam, DO., et al. 2009. Cell Cycle. 8: 1765-74. PMID: 19411845
- TLK1B promotes repair of DSBs via its interaction with Rad9 and Asf1. | Canfield, C., et al. 2009. BMC Mol Biol. 10: 110. PMID: 20021694
- Phosphorylation of Rad9 at serine 328 by cyclin A-Cdk2 triggers apoptosis via interfering Bcl-xL. | Zhan, Z., et al. 2012. PLoS One. 7: e44923. PMID: 23028682
- Mrc1 and Rad9 cooperate to regulate initiation and elongation of DNA replication in response to DNA damage. | Bacal, J., et al. 2018. EMBO J. 37: PMID: 30158111
- Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO sheds light on the other side of the DNA clamp. | Hara, K., et al. 2020. J Biol Chem. 295: 899-904. PMID: 31776186
- Structural basis for molecular interactions on the eukaryotic DNA sliding clamps PCNA and RAD9-RAD1-HUS1. | Hashimoto, H., et al. 2022. J Biochem. 172: 189-196. PMID: 35731009
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad9 Antibody (B-6) | sc-166457 | 200 µg/ml | $322.00 | |||
Rad9 Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-540106 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rad9 Antibody (B-6): m-IgG1 BP-HRP Bundle | sc-541906 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rad9 (B-6) Neutralizing Peptide | sc-166457 P | 100 µg/0.5 ml | $69.00 |