Rad9 Antibody (B-8): sc-74464

Rad9 Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rad9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad9 (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad9 protein plays a crucial role in the DNA damage response, particularly in regulating cell cycle checkpoints. Rad9 is primarily located in the nucleus, where Rad9 is involved in forming the Rad9-Rad1-Hus1 complex, which is essential for activating checkpoint kinase Chk1. This complex maintains genomic stability by halting the cell cycle in response to DNA damage, allowing repair mechanisms to occur before proceeding to mitosis. Rad9′s interaction with Chk1 and phosphorylation status are critical for proper G2 checkpoint functioning, preventing damaged DNA replication and reducing tumorigenesis risk. Rad9′s association with anti-apoptotic Bcl-2 family proteins suggests a role in regulating apoptosis following DNA damage, highlighting Rad9′s importance in both cell cycle control and cellular stress responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rad9 Antibody (B-8) References:

1. The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1.  |  St Onge, RP., et al. 1999. Mol Biol Cell. 10: 1985-95. PMID: 10359610
2. Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes apoptosis.  |  Komatsu, K., et al. 2000. Nat Cell Biol. 2: 1-6. PMID: 10620799
3. An essential role for Drosophila hus1 in somatic and meiotic DNA damage responses.  |  Abdu, U., et al. 2007. J Cell Sci. 120: 1042-9. PMID: 17327271
4. Rad9 protein contributes to prostate tumor progression by promoting cell migration and anoikis resistance.  |  Broustas, CG., et al. 2012. J Biol Chem. 287: 41324-33. PMID: 23066031
5. RAD9 enhances radioresistance of human prostate cancer cells through regulation of ITGB1 protein levels.  |  Broustas, CG. and Lieberman, HB. 2014. Prostate. 74: 1359-70. PMID: 25111005
6. Rad9/53BP1 promotes DNA repair via crossover recombination by limiting the Sgs1 and Mph1 helicases.  |  Ferrari, M., et al. 2020. Nat Commun. 11: 3181. PMID: 32576832
7. The chk1 pathway is required to prevent mitosis following cell-cycle arrest at 'start'.  |  Carr, AM., et al. 1995. Curr Biol. 5: 1179-90. PMID: 8548290
8. A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene.  |  Lieberman, HB., et al. 1996. Proc Natl Acad Sci U S A. 93: 13890-5. PMID: 8943031
9. Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation.  |  O'Connell, MJ., et al. 1997. EMBO J. 16: 545-54. PMID: 9034337
10. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.  |  Sanchez, Y., et al. 1997. Science. 277: 1497-501. PMID: 9278511
11. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.  |  Peng, CY., et al. 1997. Science. 277: 1501-5. PMID: 9278512
12. Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage.  |  Kostrub, CF., et al. 1998. EMBO J. 17: 2055-66. PMID: 9524127

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad9 Antibody (B-8) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad9 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad9 Antibody (B-8) Specifications

Alternate Product Name: Rad9

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-389

Epitope Target: Rad9

Epitope Species: mouse

Product Reactivity/Detection: Rad9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Rad9 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin