Rad9 Antibody (A-4): sc-74463
- Rad9 Antibody (A-4) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 1-389 representing full length Rad9 of mouse origin
- recommended for detection of Rad9 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Rad9 Antibody (A-4) for WB applications. This reagent is now offered in a bundle with Rad9 Antibody (A-4) (see ordering information below).
Rad9 Antibody (A-4) is a mouse monoclonal IgG2b antibody that detects Rad9 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad9 plays a critical role in the DNA damage response by functioning as a key component of the G2 phase DNA damage checkpoint, where Rad9 is involved in the signal transduction cascade that regulates cell cycle arrest. Rad9 is primarily located in the nucleus, where Rad9 associates with other checkpoint control proteins, including Rad1 and Hus1, forming a complex that is essential for maintaining genomic stability. Nuclear localization of Rad9 is crucial, as this positioning allows for rapid response to DNA damage, facilitating Cdc25C phosphorylation and subsequent inhibition of mitosis, thereby preventing damaged DNA propagation. Rad9 is highly phosphorylated in vivo, which enhances Rad9 interaction with anti-apoptotic Bcl-2 family proteins, such as Bcl-2 and Bcl-xL, while Rad9 does not interact with pro-apoptotic proteins like Bax and Bad. Rad9′s ability to modulate apoptosis in response to DNA damage underscores Rad9′s importance not only in cell cycle regulation but also in cellular responses to stress, making Rad9 a vital target for research in cancer biology and therapeutic development.
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Rad9 Antibody (A-4) References:
- Association of Rad9 with double-strand breaks through a Mec1-dependent mechanism. | Naiki, T., et al. 2004. Mol Cell Biol. 24: 3277-85. PMID: 15060150
- A novel function for human Rad9 protein as a transcriptional activator of gene expression. | Lieberman, HB. and Yin, Y. 2004. Cell Cycle. 3: 1008-10. PMID: 15280665
- Rad9, an evolutionarily conserved gene with multiple functions for preserving genomic integrity. | Lieberman, HB. 2006. J Cell Biochem. 97: 690-7. PMID: 16365875
- The role of RAD9 in tumorigenesis. | Lieberman, HB., et al. 2011. J Mol Cell Biol. 3: 39-43. PMID: 21278450
- Regulation of NEIL1 protein abundance by RAD9 is important for efficient base excision repair. | Panigrahi, SK., et al. 2015. Nucleic Acids Res. 43: 4531-46. PMID: 25873625
- p53 and RAD9, the DNA Damage Response, and Regulation of Transcription Networks. | Lieberman, HB., et al. 2017. Radiat Res. 187: 424-432. PMID: 28140789
- A cell cycle-independent mode of the Rad9-Dpb11 interaction is induced by DNA damage. | di Cicco, G., et al. 2017. Sci Rep. 7: 11650. PMID: 28912563
- Rad9/53BP1 protects stalled replication forks from degradation in Mec1/ATR-defective cells. | Villa, M., et al. 2018. EMBO Rep. 19: 351-367. PMID: 29301856
- FOXP1 and NDRG1 act differentially as downstream effectors of RAD9-mediated prostate cancer cell functions. | Panigrahi, SK., et al. 2021. Cell Signal. 86: 110091. PMID: 34298089
- DNA binding by the Rad9A subunit of the Rad9-Rad1-Hus1 complex. | Hwang, BJ., et al. 2022. PLoS One. 17: e0272645. PMID: 35939452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad9 Antibody (A-4) | sc-74463 | 200 µg/ml | $322.00 | |||
Rad9 Antibody (A-4): m-IgG2b BP-HRP Bundle | sc-548461 | 200 µg Ab; 10 µg BP | $361.00 |