Rad50 Antibody (G-2): sc-74460

Rad50 Antibody (G-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rad50 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rad50 antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad50 plays a crucial role in DNA double-strand break repair (DSBR) pathway, which is essential for maintaining genomic stability and preventing cell death, mutations, or neoplastic transformation. Rad50 functions as part of a protein complex that recognizes and binds to DNA breaks, facilitating recruitment of other repair proteins such as Rad51 and MRE11. This interaction is vital for homologous recombination repair process, which ensures accurate repair of DNA damage. Rad50′s ability to form a stable complex with Rad51 and BRCA1/BRCA2 highlights its importance in cellular response to DNA damage, as mutations in these pathways can lead to genomic instability and cancer. Multiple format options for Rad50 (G-2) antibody enable versatile applications in research, helping scientists explore intricate mechanisms of DNA repair and implications in cancer biology.

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Rad50 Antibody (G-2) References:

1. Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts.  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
2. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre.  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
3. In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination.  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
4. In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair.  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
5. The human Rap1 protein complex and modulation of telomere length.  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
6. DNA annealing mediated by Rad52 and Rad59 proteins.  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
7. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.  |  Bekker-Jensen, S., et al. 2006. J Cell Biol. 173: 195-206. PMID: 16618811
8. Overexpression of RAD50 is the Marker of Poor Prognosis and Drug Resistance in Breast Cancer Patients.  |  Karamat, U. and Ejaz, S. 2021. Curr Cancer Drug Targets. 21: 163-176. PMID: 33038913
9. Bone Marrow Failure and Immunodeficiency Associated with Human RAD50 Variants.  |  Takagi, M., et al. 2023. J Clin Immunol. 43: 2136-2145. PMID: 37794136
10. Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.  |  Pizzul, P., et al. 2024. Nucleic Acids Res. 52: 2355-2371. PMID: 38180815
11. Deficiency in epithelium RAD50 aggravates UC via IL-6-mediated JAK1/2-STAT3 signaling and promotes development of colitis-associated cancer in mice.  |  Zhang, J., et al. 2024. J Crohns Colitis.. PMID: 39207300
12. Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae.  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad50 Antibody (G-2) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad50 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad50 Antibody (G-2) Specifications

Alternate Product Name: RAD50

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1013-1312

Epitope Target: Rad50

Epitope Species: human

Product Reactivity/Detection: Rad50

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Rad50 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin