Anti-Rad23A Antibody (D-6) recommended for detection of Rad23A of human origin by WB, IP, IF and ELISA

Anti-Rad23A Antibody (D-6): sc-365669

Anti-Rad23A Antibody (D-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-Rad23A Antibody (D-6) is a mouse monoclonal IgG1 κ Rad23A antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 81-167 mapping within an internal region of Rad23A of human origin
    • Rad23A Antibody (D-6) is recommended for detection of Rad23A of human origin by WB, IP, IF and ELISA
    • Anti-Rad23A Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rad23A (D-6): sc-365669.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad23A Antibody (D-6) for WB applications. These reagents are now offered in bundles with Rad23A Antibody (D-6) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-365669: Rad23A (D-6) monoclonal antibody?

Asked by: jenniferc
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good antibody for cell lines such HeLa SSC25 Good antibody for cell lines such HeLa SSC25 and HEK293s. Was able to detect Rad23A from human keratinocytes but barely detectable in primary mouse keratinocytes. However was able to detect Rad23A from mouse tissues (epidermis l, lung etc) ie antibody can detect mouse Rad23A. Blots were clean
Date published: 2015-10-05
Rated 5 out of 5 by from Crisp band recognized in WB using HeLa Crisp band recognized in WB using HeLa, Jurkat, K-562 and HL-60 nuclear extracts and SHP-77 whole cell lysate. -SCBT QC
Date published: 2015-02-06
Rated 5 out of 5 by from Good WB data Good WB data, band of interest seen using HeLa and SHP-77 whole cell lysates. -SCBT QC
Date published: 2013-04-02
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