Rad23A Antibody (D-6): sc-365669
- Rad23A Antibody (D-6) is a mouse monoclonal IgG1 κ Rad23A antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 81-167 mapping within an internal region of Rad23A of human origin
- Rad23A Antibody (D-6) is recommended for detection of Rad23A of human origin by WB, IP, IF and ELISA
- Anti-Rad23A Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad23A Antibody (D-6) for WB applications. These reagents are now offered in bundles with Rad23A Antibody (D-6) (see ordering information below).
Rad23A Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rad23A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad23A (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rad23A protein plays a critical role in nucleotide excision repair (NER), a vital cellular mechanism that removes DNA lesions caused by ultraviolet light and other forms of damage. Rad23A protein is essential for maintaining genomic stability, as Rad23A helps ensure damaged DNA is accurately repaired, thereby preventing mutations that could lead to cancer and other diseases. Rad23A protein interacts with the 26S proteasome, facilitating the degradation of damaged proteins and contributing to cellular homeostasis. Rad23A protein′s structure includes an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain, both of which are crucial for function and are conserved across species from yeast to humans. These domains allow Rad23A protein to effectively mediate repair processes and regulate proteasome activity, highlighting Rad23A′s importance in cellular responses to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rad23A Antibody (D-6) References:
- Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination. | Elder, RT., et al. 2002. Nucleic Acids Res. 30: 581-91. PMID: 11788722
- A novel regulation mechanism of DNA repair by damage-induced and RAD23-dependent stabilization of xeroderma pigmentosum group C protein. | Ng, JM., et al. 2003. Genes Dev. 17: 1630-45. PMID: 12815074
- Ubiquitin recognition by the DNA repair protein hHR23a. | Wang, Q., et al. 2003. Biochemistry. 42: 13529-35. PMID: 14621999
- Structure of the XPC binding domain of hHR23A reveals hydrophobic patches for protein interaction. | Kamionka, M. and Feigon, J. 2004. Protein Sci. 13: 2370-7. PMID: 15322280
- Relative levels of the two mammalian Rad23 homologs determine composition and stability of the xeroderma pigmentosum group C protein complex. | Okuda, Y., et al. 2004. DNA Repair (Amst). 3: 1285-95. PMID: 15336624
- The UBA2 domain functions as an intrinsic stabilization signal that protects Rad23 from proteasomal degradation. | Heessen, S., et al. 2005. Mol Cell. 18: 225-35. PMID: 15837425
- Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B. | Kim, B., et al. 2005. FEBS J. 272: 2467-76. PMID: 15885096
- HHR23A, a human homolog of Saccharomyces cerevisiae Rad23, regulates xeroderma pigmentosum C protein and is required for nucleotide excision repair. | Hsieh, HC., et al. 2005. Biochem Biophys Res Commun. 335: 181-7. PMID: 16105547
- Evidence for distinct functions for human DNA repair factors hHR23A and hHR23B. | Chen, L. and Madura, K. 2006. FEBS Lett. 580: 3401-8. PMID: 16712842
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad23A Antibody (D-6) | sc-365669 | 200 µg/ml | $316.00 | |||
Rad23A Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-529433 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rad23A Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-522321 | 200 µg Ab, 40 µg BP | $354.00 | |||
Rad23A Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-543516 | 200 µg Ab; 20 µg BP | $354.00 | |||
Rad23A Antibody (D-6) AC | sc-365669 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Rad23A Antibody (D-6) HRP | sc-365669 HRP | 200 µg/ml | $316.00 | |||
Rad23A Antibody (D-6) FITC | sc-365669 FITC | 200 µg/ml | $330.00 | |||
Rad23A Antibody (D-6) PE | sc-365669 PE | 200 µg/ml | $343.00 | |||
Rad23A Antibody (D-6) Alexa Fluor® 488 | sc-365669 AF488 | 200 µg/ml | $357.00 | |||
Rad23A Antibody (D-6) Alexa Fluor® 546 | sc-365669 AF546 | 200 µg/ml | $357.00 | |||
Rad23A Antibody (D-6) Alexa Fluor® 594 | sc-365669 AF594 | 200 µg/ml | $357.00 | |||
Rad23A Antibody (D-6) Alexa Fluor® 647 | sc-365669 AF647 | 200 µg/ml | $357.00 | |||
Rad23A Antibody (D-6) Alexa Fluor® 680 | sc-365669 AF680 | 200 µg/ml | $357.00 | |||
Rad23A Antibody (D-6) Alexa Fluor® 790 | sc-365669 AF790 | 200 µg/ml | $357.00 |