Rabex-5 Antibody (C-4): sc-166049

Rabex-5 Antibody (C-4) is a mouse monoclonal IgG2b antibody that detects Rabex-5 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Rabex-5, also known as RABGEF1, RAP1, or RABAPTIN-5-associated exchange factor for Rab 5, plays a crucial role as a guanine nucleotide exchange factor that facilitates the activation of Rab 5, a key regulator of intracellular vesicle trafficking. Rabex-5 is primarily localized in the cytoplasm and associates with early endosomes, where Rabex-5 is essential for the proper functioning of endocytic pathways. The structure of Rabex-5 includes an N-terminal zinc finger domain, a GEF domain, an early endosomal targeting (EET) domain, and a coiled-coil domain, with the EET domain being particularly important for interaction with early endosomes and the activation of Rab 5. Notably, truncated forms of Rabex-5 lacking the EET domain can still function through association with RABAPTIN-5, highlighting Rabex-5′s versatility in endosomal targeting. The Rabex-5/RABAPTIN-5 complex is critical for recruitment to early endosomes in a Rab 5-dependent manner, as recruitment relies on the binding of Rab5-GTP to RABAPTIN-5, thereby underscoring Rabex-5′s importance in maintaining cellular homeostasis and facilitating intracellular transport processes. anti-Rabex-5 antibody (C-4) is an invaluable tool for researchers studying the dynamics of endosomal trafficking and the molecular mechanisms underlying various cellular functions.

The preferred Rabex-5 antibody is Rabex-5 Antibody (A-7): sc-166611.