Rabex-5 Antibody (A-7): sc-166611
- Rabex-5 Antibody (A-7) is a mouse monoclonal IgG2b κ Rabex-5 antibody provided at 200 µg/ml
- raised against amino acids 421-708 mapping at the C-terminus of Rabex-5 of human origin
- Rabex-5 Antibody (A-7) is recommended for detection of Rabex-5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Rabex-5 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Rabex-5 Antibody (A-7) for WB applications. This reagent is now offered in a bundle with Rabex-5 Antibody (A-7) (see ordering information below).
QUICK LINKS
SEE ALSO...
Rabex-5 Antibody (A-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Rabex-5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rabex-5 (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rabex-5, also known as RABGEF1, RAP1, or RABAPTIN-5-associated exchange factor for Rab 5, functions as a guanine nucleotide exchange factor (GEF) that plays a crucial role in regulating intracellular trafficking. Rabex-5 is primarily located in the cytoplasm and associates with early endosomes, where Rabex-5 facilitates the activation of Rab 5, a key player in endosomal dynamics. The structure of Rabex-5 includes an N-terminal zinc finger domain, a GEF domain, an early endosomal targeting (EET) domain, and a coiled-coil domain, with the EET domain being particularly important for localization and function. Rabex-5′s interaction with early endosomes is vital for proper sorting and transport of vesicles within the cell, ensuring cellular processes such as nutrient uptake and receptor recycling occur efficiently. Notably, truncated forms of Rabex-5 lacking the EET domain can still associate with RABAPTIN-5, allowing for some functional redundancy in endosomal targeting. The Rabex-5/RABAPTIN-5 complex is essential for recruiting Rab 5 to early endosomes, highlighting the importance of protein-protein interactions in cellular trafficking pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rabex-5 Antibody (A-7) References:
- Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5. | Delprato, A., et al. 2004. Cell. 118: 607-17. PMID: 15339665
- The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain. | Mattera, R., et al. 2006. J Biol Chem. 281: 6874-83. PMID: 16407276
- Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. | Lee, S., et al. 2006. Nat Struct Mol Biol. 13: 264-71. PMID: 16462746
- Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. | Penengo, L., et al. 2006. Cell. 124: 1183-95. PMID: 16499958
- RabGEF1, a negative regulator of Ras signalling, mast cell activation and skin inflammation. | Tam, SY., et al. 2005. Novartis Found Symp. 271: 115-24; discussion 124-30, 145-51. PMID: 16605131
- Evolutionarily conserved structural and functional roles of the FYVE domain. | Hayakawa, A., et al. 2007. Biochem Soc Symp. 95-105. PMID: 17233583
- Structural basis for Rab GTPase activation by VPS9 domain exchange factors. | Delprato, A. and Lambright, DG. 2007. Nat Struct Mol Biol. 14: 406-12. PMID: 17450153
- Thyrotropin activates guanosine 5'-diphosphate/guanosine 5'-triphosphate exchange on the rate-limiting endocytic catalyst, Rab5a, in human thyrocytes in vivo and in vitro. | van den Hove, MF., et al. 2007. J Clin Endocrinol Metab. 92: 2803-10. PMID: 17473071
- Rabaptin-5-independent membrane targeting and Rab5 activation by Rabex-5 in the cell. | Zhu, H., et al. 2007. Mol Biol Cell. 18: 4119-28. PMID: 17699593
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rabex-5 Antibody (A-7) | sc-166611 | 200 µg/ml | $322.00 | |||
Rabex-5 Antibody (A-7): m-IgG2b BP-HRP Bundle | sc-548678 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rabex-5 Antibody (A-7) AC | sc-166611 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rabex-5 Antibody (A-7) HRP | sc-166611 HRP | 200 µg/ml | $322.00 | |||
Rabex-5 Antibody (A-7) FITC | sc-166611 FITC | 200 µg/ml | $336.00 | |||
Rabex-5 Antibody (A-7) PE | sc-166611 PE | 200 µg/ml | $349.00 | |||
Rabex-5 Antibody (A-7) Alexa Fluor® 488 | sc-166611 AF488 | 200 µg/ml | $364.00 | |||
Rabex-5 Antibody (A-7) Alexa Fluor® 546 | sc-166611 AF546 | 200 µg/ml | $364.00 | |||
Rabex-5 Antibody (A-7) Alexa Fluor® 594 | sc-166611 AF594 | 200 µg/ml | $364.00 | |||
Rabex-5 Antibody (A-7) Alexa Fluor® 647 | sc-166611 AF647 | 200 µg/ml | $364.00 | |||
Rabex-5 Antibody (A-7) Alexa Fluor® 680 | sc-166611 AF680 | 200 µg/ml | $364.00 | |||
Rabex-5 Antibody (A-7) Alexa Fluor® 790 | sc-166611 AF790 | 200 µg/ml | $364.00 |