Rab GDI β Antibody (D-2): sc-515143

Rab GDI β Antibody (D-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rab GDI β in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rab GDI β antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab GDI β, a member of the Rab family of Ras-related small GTP-binding proteins, plays a crucial role in regulating intracellular vesicle trafficking by forming a soluble complex with Rab proteins, thereby inhibiting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). This function is vital for maintaining proper vesicle localization and transport within cells, which is essential for various cellular processes, including secretion, endocytosis, and organelle dynamics. Rab GDI β genes are ubiquitously expressed across different tissues, highlighting their importance in cellular function. The evolutionary conservation of Rab GDI protein sequences across three eukaryotic kingdoms underscores their fundamental role in cellular biology.

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Rab GDI β Antibody (D-2) References:

1. The amphioxus rab GDP-dissociation inhibitor (GDI) gene is neural-specific: implications for the evolution of chordate rab GDI genes.  |  Sedlacek, Z., et al. 1999. Mol Biol Evol. 16: 1231-7. PMID: 10486978
2. Reduction of nucleoside diphosphate kinase B, Rab GDP-dissociation inhibitor beta and histidine triad nucleotide-binding protein in fetal Down syndrome brain.  |  Weitzdoerfer, R., et al. 2001. J Neural Transm Suppl. 347-59. PMID: 11771757
3. Beta8 integrin binds Rho GDP dissociation inhibitor-1 and activates Rac1 to inhibit mesangial cell myofibroblast differentiation.  |  Lakhe-Reddy, S., et al. 2006. J Biol Chem. 281: 19688-99. PMID: 16690620
4. Proteome analysis of human substantia nigra in Parkinson's disease.  |  Werner, CJ., et al. 2008. Proteome Sci. 6: 8. PMID: 18275612
5. Comparative proteomic analysis of anti-cancer mechanism by periplocin treatment in lung cancer cells.  |  Lu, Z., et al. 2014. Cell Physiol Biochem. 33: 859-68. PMID: 24685647
6. Bioinformatics analysis of Rab GDP dissociation inhibitor beta and its expression in non-small cell lung cancer.  |  Ming, Z., et al. 2014. Diagn Pathol. 9: 201. PMID: 25367783
7. Protein tyrosine phosphatase-PEST and β8 integrin regulate spatiotemporal patterns of RhoGDI1 activation in migrating cells.  |  Lee, HS., et al. 2015. Mol Cell Biol. 35: 1401-13. PMID: 25666508
8. Expression patterns of two human genes coding for different rab GDP-dissociation inhibitors (GDIs), extremely conserved proteins involved in cellular transport.  |  Bächner, D., et al. 1995. Hum Mol Genet. 4: 701-8. PMID: 7543319
9. Cloning of a brain-type isoform of human Rab GDI and its expression in human neuroblastoma cell lines and tumor specimens.  |  Nishimura, N., et al. 1995. Cancer Res. 55: 5445-50. PMID: 7585614
10. Purification and characterization of Rab GDI beta from rat brain.  |  Araki, K., et al. 1995. Biochem Biophys Res Commun. 211: 296-305. PMID: 7779099
11. Molecular cloning and characterization of two rab GDI species from rat brain: brain-specific and ubiquitous types.  |  Nishimura, N., et al. 1994. J Biol Chem. 269: 14191-8. PMID: 8188702
12. N-ethylmaleimide-sensitive factor-dependent alpha-SNAP release, an early event in the docking/fusion process, is not regulated by Rab GTPases.  |  Colombo, MI., et al. 1998. J Biol Chem. 273: 1334-8. PMID: 9430666

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rab GDI β Antibody (D-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Rab GDI β Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rab GDI β Antibody (D-2) Specifications

Alternate Product Name: Rab GDI β

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 376-407

Epitope Target: Rab GDI β

Epitope Species: human

Product Reactivity/Detection: Rab GDI β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Rab GDI β Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Rab GDI α/β antibody is Rab GDI α/β Antibody (E-5): sc-374649.