Rab GDI α/β Antibody (E-5): sc-374649

Rab GDI α/β Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rab GDI alpha / beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Rab GDI α/β antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rab GDP dissociation inhibitor (Rab GDI) plays a crucial role in intracellular vesicle trafficking by forming a soluble complex with Rab proteins, thereby inhibiting the exchange of GDP for GTP. This regulation is vital for maintaining proper function of Rab proteins, which are essential for vesicle transport within cells. Rab GDI α, also known as XAP-4, is predominantly expressed in neural and sensory tissues, suggesting a specialized role in neural signal transmission. The presence of two major isoforms, Rab GDI α and Rab GDI β, with Rab GDI β being ubiquitously expressed, highlights evolutionary conservation across eukaryotic kingdoms, underscoring importance in cellular processes. Rab GDI α/β (E-5) monoclonal antibody serves as an invaluable tool for researchers studying dynamics of vesicle trafficking and specific functions of Rab proteins in various biological contexts.

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Rab GDI α/β Antibody (E-5) References:

1. The amphioxus rab GDP-dissociation inhibitor (GDI) gene is neural-specific: implications for the evolution of chordate rab GDI genes.  |  Sedlacek, Z., et al. 1999. Mol Biol Evol. 16: 1231-7. PMID: 10486978
2. Different Rab GTPases associate preferentially with alpha or beta GDP-dissociation inhibitors.  |  Erdman, RA. and Maltese, WA. 2001. Biochem Biophys Res Commun. 282: 4-9. PMID: 11263962
3. Proteome analysis of human substantia nigra in Parkinson's disease.  |  Werner, CJ., et al. 2008. Proteome Sci. 6: 8. PMID: 18275612
4. Levels of the Rab GDP dissociation inhibitor (GDI) are altered in the prenatal restrain stress mouse model of schizophrenia and are differentially regulated by the mGlu2/3 receptor agonists, LY379268 and LY354740.  |  Orlando, R., et al. 2014. Neuropharmacology. 86: 133-44. PMID: 25063582
5. Metastasising Fibroblasts Show an HDAC6-Dependent Increase in Migration Speed and Loss of Directionality Linked to Major Changes in the Vimentin Interactome.  |  Evans, CA., et al. 2022. Int J Mol Sci. 23: PMID: 35216078
6. Expression patterns of two human genes coding for different rab GDP-dissociation inhibitors (GDIs), extremely conserved proteins involved in cellular transport.  |  Bächner, D., et al. 1995. Hum Mol Genet. 4: 701-8. PMID: 7543319
7. Cloning of a brain-type isoform of human Rab GDI and its expression in human neuroblastoma cell lines and tumor specimens.  |  Nishimura, N., et al. 1995. Cancer Res. 55: 5445-50. PMID: 7585614
8. Purification and characterization of Rab GDI beta from rat brain.  |  Araki, K., et al. 1995. Biochem Biophys Res Commun. 211: 296-305. PMID: 7779099
9. Molecular cloning and characterization of two rab GDI species from rat brain: brain-specific and ubiquitous types.  |  Nishimura, N., et al. 1994. J Biol Chem. 269: 14191-8. PMID: 8188702
10. The human rab GDI beta gene with long retroposon-rich introns maps to 10p15 and its pseudogene to 7p11-p13.  |  Sedlacek, Z., et al. 1998. Mamm Genome. 9: 78-80. PMID: 9434952

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rab GDI α/β Antibody (E-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Rab GDI α/β Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rab GDI α/β Antibody (E-5) Specifications

Alternate Product Name: Rab GDI α/β

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: Rab GDI α

Epitope Species: human

Product Reactivity/Detection: Rab GDI α, Rab GDI β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Rab GDI α/β Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin